NFIB Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81000
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Rat (99%). Backed by our 100% Guarantee.
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Cited:
Chemotaxis, IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VKSGVFNVSELVRVSRTPITQGTGVNFPIGEIPSQPYYHDMNSGVNLQRSLSSPPSSKRPKTISIDENMEPSPTGDFYPSPSSPAAGSRTWHERDQDMSSPTTMKKPEKPLFSSASPQDSSPRLSTFPQHHHPGIPGVAHSVISTRTP
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 24553933).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NFIB Antibody
![Immunocytochemistry/ Immunofluorescence: NFIB Antibody [NBP1-81000]](https://resources.bio-techne.com/images/products/NFIB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81000-img0009.jpg "Immunocytochemistry/ Immunofluorescence: NFIB Antibody [NBP1-81000]")
Immunocytochemistry/ Immunofluorescence: NFIB Antibody [NBP1-81000]
Immunocytochemistry/Immunofluorescence: NFIB Antibody [NBP1-81000] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center. Antibody staining is shown in green.![Western Blot: NFIB Antibody [NBP1-81000]](https://resources.bio-techne.com/images/products/NFIB-Antibody-Western-Blot-NBP1-81000-img0007.jpg "Western Blot: NFIB Antibody [NBP1-81000]")
Western Blot: NFIB Antibody [NBP1-81000]
Western Blot: NFIB Antibody [NBP1-81000] - Analysis in human cell line SH-SY5Y.Applications for NFIB Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Reported in scientific literature (PMID: 24801505)
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
Reported in scientif literature (PMID: 26021864)
Western Blot
0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: PFA/Triton X-100
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NFIB
Alternate Names
CCAAT-box-binding transcription factor, CTF, HMGIC/NFIB, NF-I/B, NFI-B, NFIB2, NFIB3, NFI-RED, nuclear factor 1 B-type, Nuclear factor 1/B, nuclear factor I/BNF1-B, TGGCA-binding protein
Entrez Gene IDs
4781 (Human)
Gene Symbol
NFIB
UniProt
Additional NFIB Products
Product Documents for NFIB Antibody
Product Specific Notices for NFIB Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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