NFIB Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-04121
![Immunoprecipitation: NFIB Antibody [NBP2-04121]](https://resources.bio-techne.com/images/products/NFIB-Antibody-Immunoprecipitation-NBP2-04121-img0002.jpg "Immunoprecipitation: NFIB Antibody [NBP2-04121]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 225 and 275 of human Nuclear Factor I/B using the numbering given in entry NP_005587.2 (GeneID 4781).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NFIB Antibody
Immunoprecipitation: NFIB Antibody [NBP2-04121]
Immunoprecipitation: NFIB Antibody [NBP2-04121] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFIB antibody NBP2-04121 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFIB was also immunoprecipitated by rabbit anti-NFIB antibody NBP2-04122, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).Applications for NFIB Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 mcg/mg
Western Blot
1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NFIB
Alternate Names
CCAAT-box-binding transcription factor, CTF, HMGIC/NFIB, NF-I/B, NFI-B, NFIB2, NFIB3, NFI-RED, nuclear factor 1 B-type, Nuclear factor 1/B, nuclear factor I/BNF1-B, TGGCA-binding protein
Gene Symbol
NFIB
UniProt
Additional NFIB Products
Product Documents for NFIB Antibody
Product Specific Notices for NFIB Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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