Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NFIB Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 325 and 375 of human Nuclear Factor I/B using the numbering given in entry NP_005587.2 (GeneID 4781).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFIB Antibody

Immunoprecipitation: NFIB Antibody [NBP2-04122]

Immunoprecipitation: NFIB Antibody [NBP2-04122]

Immunoprecipitation: NFIB Antibody [NBP2-04122] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFIB antibody NBP2-04121 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFIB was also immunoprecipitated by rabbit anti-NFIB antibody NBP2-04122, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Applications for NFIB Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NFIB

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Alternate Names

CCAAT-box-binding transcription factor, CTF, HMGIC/NFIB, NF-I/B, NFI-B, NFIB2, NFIB3, NFI-RED, nuclear factor 1 B-type, Nuclear factor 1/B, nuclear factor I/BNF1-B, TGGCA-binding protein

Gene Symbol

NFIB

UniProt

NP_005587.2 (Human)

Additional NFIB Products

Product Documents for NFIB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NFIB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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