NFIL3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-842
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-RLIATQPISASDSG corresponding to C-Terminus according to NP_005375.2.
Reactivity Notes
Expected from sequence similarity: Mouse, Rat
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for NFIL3 Antibody
![Western Blot: NFIL3 Antibody [NB100-842]](https://resources.bio-techne.com/images/products/NFIL3-Antibody-Western-Blot-NB100-842-img0003.jpg "Western Blot: NFIL3 Antibody [NB100-842]")
Western Blot: NFIL3 Antibody [NB100-842]
Western Blot: NFIL3 Antibody [NB100-842] - Analysis of NFIL3 in HepG2 lysate using NB100-842 at 0.5ug/ml (RIPA buffer, 20ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.![Flow Cytometry: NFIL3 Antibody [NB100-842]](https://resources.bio-techne.com/images/products/NFIL3-Antibody-Flow-Cytometry-NB100-842-img0004.jpg "Flow Cytometry: NFIL3 Antibody [NB100-842]")
Flow Cytometry: NFIL3 Antibody [NB100-842]
Flow Cytometry: NFIL3 Antibody [NB100-842] - analysis of paraformaldehyde fixed Human peripheral blood mononuclear cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: UnimmunizedApplications for NFIL3 Antibody
Application
Recommended Usage
Flow Cytometry
0.5-2ug/ml
Peptide ELISA
Detection limit 1:8000
Western Blot
1:1000
Application Notes
Western blot: Approx. 60kDa band observed in nuclear lysates of cell line Jurkat and HeLa (calculated MW of 51.5kDa according to NP_005375.2). The observed molecular weight corresponds to different antibodies from other commercial sources. An additional band of 30kDa was observed in lysates of cell line HeLa, which was completely blocked with the immunizing peptide. Primary incubation 1 hour at room temperature
Flow Cytometry: Flow cytometric analysis of Human peripheral blood mononuclear cells
Flow Cytometry: Flow cytometric analysis of Human peripheral blood mononuclear cells
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NFIL3/E4BP4
Long Name
Nuclear Factor, Interleukin 3 Regulated
Alternate Names
E4BP4, IL3BP1, NF-IL3A
Gene Symbol
NFIL3
UniProt
Additional NFIL3/E4BP4 Products
Product Documents for NFIL3 Antibody
Product Specific Notices for NFIL3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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