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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Summary for NFIL3 Antibody

Immunogen

The immunogen for this product maps to a region between residue 412 and 462 of human E4 promoter binding-protein 4 using the numbering given in entry NP_005375.2 (GeneID 4783).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFIL3 Antibody

Western Blot: NFIL3 Antibody [NBP1-41077]

Western Blot: NFIL3 Antibody [NBP1-41077]

Western Blot: NFIL3 Antibody [NBP1-41077] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-E4BP4 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E4BP4 was also immunoprecipitated by another rabbit anti-E4BP4 antibody, which recognizes an upstream epitope.

Applications for NFIL3 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NFIL3/E4BP4

NFIL3 is a basic leucine zipper transcription factor which activates IL3 gene expression and can also repress transcription.

Long Name

Nuclear Factor, Interleukin 3 Regulated

Alternate Names

E4BP4, IL3BP1, NF-IL3A

Entrez Gene IDs

4783 (Human)

Gene Symbol

NFIL3

Additional NFIL3/E4BP4 Products

Product Documents for NFIL3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NFIL3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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