NFIX Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15039
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Xenopus (90%). Backed by our 100% Guarantee.
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Chemotaxis, IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human NFIX. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (90%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NFIX Antibody
![Immunocytochemistry/ Immunofluorescence: NFIX Antibody [NBP2-15039]](https://resources.bio-techne.com/images/products/NFIX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15039-img0010.jpg "Immunocytochemistry/ Immunofluorescence: NFIX Antibody [NBP2-15039]")
Immunocytochemistry/ Immunofluorescence: NFIX Antibody [NBP2-15039]
Immunocytochemistry/Immunofluorescence: NFIX Antibody [NBP2-15039] - Nfix is mainly expressed by anti-inflammatory MPs. Percentage of F4/80+ MPs positive for Nfix in Tibialis Anterior muscles (TA) of WT mice injected by CTX at D2, D4 and D7, post-injury. Immunostaining for F4/80 (green), Nfix (red) and DAPI (blue) at D4 and D7 after CTX injection. Results are means +/- SEM of at least three independent experiments. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2073-4409/9/3/708) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-15039] -
Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-15039] - Mouse hippocampus.NFIX stained by NFIX antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-15039] -
Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-15039] - Mouse hippocampus.NFIX stained by NFIX antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for NFIX Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Reported in scientific literature (PMID: 30266829).
Immunocytochemistry/ Immunofluorescence
Reported in scientific literature (PMID: 30266829).
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
Reported in scientific literature (PMID: 30266829).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NFIX
Alternate Names
CTF, NF1ACCAAT-box-binding transcription factor, NF1-X, NF-I/X, NFI-X, nuclear factor 1 X-type, Nuclear factor 1/X, Nuclear factor I/X, nuclear factor I/X (CCAAT-binding transcription factor), TGGCA-binding protein
Gene Symbol
NFIX
UniProt
Additional NFIX Products
Product Documents for NFIX Antibody
Product Specific Notices for NFIX Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.