NFIX Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-58904

Novus Biologicals, part of Bio-Techne
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NBP2-58904
NBP2-58904-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NFIX Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: SPSYYNINQVTLGRRSITSPPSTSTTKRPKSIDDSEMESPVDDVFYPGTGRSPAAGSSQSSGWPNDVDAGSPRATASALHFPSTSIIQQSSPYFTHPTIRYHHHHGQDSLKEFVQFVCSDGSGQATGQHSQRQAPPLPTGLSASD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFIX Antibody

Western Blot: NFIX Antibody [NBP2-58904]

Western Blot: NFIX Antibody [NBP2-58904]

Western Blot: NFIX Antibody [NBP2-58904] - Analysis in human cell line U-138MG.
Immunocytochemistry/ Immunofluorescence: NFIX Antibody [NBP2-58904]

Immunocytochemistry/ Immunofluorescence: NFIX Antibody [NBP2-58904]

Immunocytochemistry/Immunofluorescence: NFIX Antibody [NBP2-58904] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-58904]

Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-58904]

Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-58904] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.
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Applications for NFIX Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFIX

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Alternate Names

CTF, NF1ACCAAT-box-binding transcription factor, NF1-X, NF-I/X, NFI-X, nuclear factor 1 X-type, Nuclear factor 1/X, Nuclear factor I/X, nuclear factor I/X (CCAAT-binding transcription factor), TGGCA-binding protein

Gene Symbol

NFIX

Additional NFIX Products

Product Documents for NFIX Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NFIX Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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