NFkB p105/p50 Antibody (2J10D7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56583

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Rat, Rabbit

Cited:

Human, Rat, Rabbit

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2J10D7

Format

BSA Free

Concentration

1.0 mg/ml

View all NFkB p105/p50 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 150-200 of human NF-kB (p50) was used as the immunogen.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%). Rat reactivity reported in scientific literature (PMID:31850807).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for NFkB p105/p50 Antibody (2J10D7) - BSA Free

Western Blot: NFkB p105/p50 Antibody (2J10D7)BSA Free [NB100-56583]

Western Blot: NFkB p105/p50 Antibody (2J10D7)BSA Free [NB100-56583]

Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.
Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - IHC-P of rabbit aorta using NB100-56583. Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin. Submitted via verified customer review
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.
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Applications for NFkB p105/p50 Antibody (2J10D7) - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

2-5 ug/ml

Reviewed Applications

Read 1 review rated 5 using NB100-56583 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFkB p105/p50

Principal Names: Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1); DNA-binding factor KBF1; EBP-1; NFKB-p50; Official Gene Symbol: NFKB1 Gene ID: 4790 (Human) Gene Map Locus: 4q24 (human) NF-kB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. It is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50), a 50KDa functional sub-unit of NF-kB, is a member of Rel protein family. It is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NF-kB factor. NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-bidning. Pathological role of NF-kB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.

Alternate Names

DKFZp686C01211, DNA binding factor KBF1, DNA-binding factor KBF1, EBP-1, KBF1, NF-kappaB, NF-kappa-B, NF-kappabeta, NFKB-p105, NFKB-p50, nuclear factor kappa-B DNA binding subunit, nuclear factor NF-kappa-B p105 subunit, nuclear factor NF-kappa-B p50 subunit, nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151, p105, p50

Entrez Gene IDs

4790 (Human)

Gene Symbol

NFKB1

UniProt

P25942

Additional NFkB p105/p50 Products

Product Documents for NFkB p105/p50 Antibody (2J10D7) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NFkB p105/p50 Antibody (2J10D7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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