NFkB p105/p50 [p Ser927] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-82069

Novus Biologicals, part of Bio-Techne
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NB100-82069
NB100-82069-0.025ml
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NFkB p105/p50 Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human NFkB p105/p50 around the phosphorylation site of serine 927 (C-D-SP-G-V).

Modification

p Ser927

Specificity

Detects endogenous level of NFkB p105/p50 only when phosphorylated at serine 927.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NFkB p105/p50 [p Ser927] Antibody

Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 [p Ser927] Antibody [NB100-82069]

Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 [p Ser927] Antibody [NB100-82069]

Immunocytochemistry/Immunofluorescence: NFkB p105/p50 [p Ser927] Antibody [NB100-82069] - Staining of methanol-fixed Hela cells using NFkB-p105(Phospho-Ser927) Antibody.

Immunohistochemistry-Paraffin:

Immunohistochemistry-Paraffin: NFkB p105/p50 [p Ser927] Antibody [NB100-82069] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-82069 (left) or the same antibody preincubated with blocking peptide(right).
Western Blot: NFkB p105/p50 [p Ser927] Antibody [NB100-82069]

Western Blot: NFkB p105/p50 [p Ser927] Antibody [NB100-82069]

Western Blot: NFkB p105/p50 [p Ser927] Antibody [NB100-82069] - Analysis of extracts from HL60 cells untreated or treated with TNF-alpha using NFkB-p105(Phospho-Ser927) antibody.

Applications for NFkB p105/p50 [p Ser927] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFkB p105/p50

NFkB is a transcription regulator that is activated by various intra and extra cellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Serine 529 is located in a negatively charged region (amino acids 422-540) that is phosphorylated in response to phorbol myristate acetate plus calcium ionophore activation. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth.

Alternate Names

DKFZp686C01211, DNA binding factor KBF1, DNA-binding factor KBF1, EBP-1, KBF1, NF-kappaB, NF-kappa-B, NF-kappabeta, NFKB-p105, NFKB-p50, nuclear factor kappa-B DNA binding subunit, nuclear factor NF-kappa-B p105 subunit, nuclear factor NF-kappa-B p50 subunit, nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151, p105, p50

Entrez Gene IDs

4790 (Human); 18033 (Mouse); 81736 (Rat)

Gene Symbol

NFKB1

UniProt

P19838

Additional NFkB p105/p50 Products

Product Documents for NFkB p105/p50 [p Ser927] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NFkB p105/p50 [p Ser927] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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