NFkB1/NFkB p105 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05867

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NFkB1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human NFkB1/NFkB p105.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for NFkB1/NFkB p105 Antibody

Western Blot: NFkB1/NFkB p105 Antibody [NBP3-05867]

Western Blot: NFkB1/NFkB p105 Antibody [NBP3-05867]

Western Blot: NFkB1/NFkB p105 Antibody [NBP3-05867] - Lane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:105 kDaObserved band size:120 kDa
Immunocytochemistry/ Immunofluorescence: NFkB1/NFkB p105 Antibody [NBP3-05867]

Immunocytochemistry/ Immunofluorescence: NFkB1/NFkB p105 Antibody [NBP3-05867]

Immunocytochemistry/Immunofluorescence: NFkB1/NFkB p105 Antibody [NBP3-05867] - Staining of NFKB1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NFKB1 polyclonal antibody (dilution ratio 1:1000) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to.

Applications for NFkB1/NFkB p105 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFkB1

Nuclear factor kappa beta (NFKB) is a transcription factor that is highly influenced by stress, free-radicals, UV light and hypoxia. Unsurprisingly, NFKB is essential for many human cancers, as it regulates the expression of many downstream proteins that regulate or modify cellular growth and development. NFKB effects on angiogenic pathways and reactive mechanisms to stress and damage are well established throughout literature. Atherosclerosis is partly attributed to the mis-regulation of NFKB in hypoxia. Arthritis is related to inflammation proteins regulated through NFKB. It also has been shown to be important in synapses in brain, leading to memory and cognitive ability or impairment. In all, NFKB is one of the fastest responding transcription factors in humans, and is likely to mediate many responsive events in diseases and injury recovery.

Long Name

Nuclear Factor Kappa-B, Subunit 1

Alternate Names

NF-kB1

Gene Symbol

NFKB1

Additional NFkB1 Products

Product Documents for NFkB1/NFkB p105 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NFkB1/NFkB p105 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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