NFkB2/NFkB p100 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87760

Novus Biologicals, part of Bio-Techne
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NBP1-87760
NBP1-87760-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NFkB2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLNAAQNTMEPPLTPPSPAGPGLSLGDTALQNLEQLLDGPEAQGSWAELAERLGLRSLVDTYRQTTSPSGSLLRSYELAGGDLAGLLEALSDMGLEEGVRLLRGPETRDKLPSTEVKEDSAYGSQSVEQEA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFkB2/NFkB p100 Antibody

Western Blot: NFkB2/NFkB p100 Antibody [NBP1-87760]

Western Blot: NFkB2/NFkB p100 Antibody [NBP1-87760]

Western Blot: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Western blot analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760]

Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760]

Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining in human appendix and skeletal muscle tissues using anti-NFKB2 antibody. Corresponding NFKB2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760]

Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760]

Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-NFKB2 antibody NBP1-87760 (A) shows similar protein distribution across tissues to independent antibody NBP1-87759 (B).
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Applications for NFkB2/NFkB p100 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:500 - 1:1000

Simple Western

1:30

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFkB2

NFkB1 (NFkB p50) and NFkB2 (NFkB p52) are class I members of the Rel/NFkB family of transcription factors that also includes RelA, c-Rel and RelB. Rel/NFkB members regulate expression of genes that participate in immune, apoptotic and oncogenic processes. Class I members lack a C-terminal transcriptional activation segment found in class II members, but contain an RHD (Rel homology domain) that mediates DNA binding, nuclear localization and dimerization. NFkB1 and NFkB2 105 and 100 kDa precursors are proteolytically cleaved to 50 and 52 kDa active subunits, respectively. NFkB is predominantly localized in the cytoplasm as a complex with inhibitory IkB proteins and is released and translocated to the nucleus after phosphorylation of IkB.

Long Name

Nuclear Factor Kappa-B, Subunit 2

Alternate Names

LYT10, NF-kB2

Gene Symbol

NFKB2

Additional NFkB2 Products

Product Documents for NFkB2/NFkB p100 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NFkB2/NFkB p100 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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