NFYA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19533

Novus Biologicals, part of Bio-Techne
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NBP2-19533
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (97%), Porcine (97%), Rhesus Macaque (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NFYA Antibodies »

Product Specifications

Immunogen

Full length human NFYA Recombinant protein.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NFYA Antibody

Western Blot: NFYA Antibody [NBP2-19533]

Western Blot: NFYA Antibody [NBP2-19533]

Western Blot: NFYA Antibody [NBP2-19533] - NF-YA is essential for lincRNA-p21 regulation by mutant P53 but not wild-type p53. The binding of p53 to NF-YA in HN6, Cal27 and HN30 cells was determined by coimmunoprecipitation. *: P < 0.05; **: P < 0.01 Image collected and cropped by CiteAb from the following publication (https://molecular-cancer.biomedcentral.com/articles/10.1186/s12943-019-0993-3) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: NFYA Antibody [NBP2-19533]

Immunocytochemistry/ Immunofluorescence: NFYA Antibody [NBP2-19533]

Immunocytochemistry/Immunofluorescence: NFYA Antibody [NBP2-19533] - NFYA antibody detects NFYA protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFYA stained by NFYA antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.
Immunohistochemistry-Paraffin: NFYA Antibody [NBP2-19533]

Immunohistochemistry-Paraffin: NFYA Antibody [NBP2-19533]

Immunohistochemistry-Paraffin: NFYA Antibody [NBP2-19533] - Immunohistochemical analysis of paraffin-embedded D54 xenograft, using antibody at 1:500 dilution.
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Applications for NFYA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NFYA

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq]

Alternate Names

CAAT box DNA-binding protein subunit A, CBF-A, CBF-B, CCAAT-binding transcription factor subunit B, FLJ11236, HAP2, HAP2 CCAAT-binding protein, NF-YACAAT-box DNA binding protein subunit A, Nuclear transcription factor Y subunit A, nuclear transcription factor Y subunit alpha, nuclear transcription factor Y, alpha, Transcription factor NF-Y, A subunit

Gene Symbol

NFYA

Additional NFYA Products

Product Documents for NFYA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NFYA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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