NFYB Antibody (6H6)
Novus Biologicals, part of Bio-Techne | Catalog # H00004801-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 6H6
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NFYB (AAH05317, 1 a.a. ~ 207 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTMDGDSSTTDASQLGISADYIGGSHYVIQPHDDTEDSMNDHEDTNGSKESFREQDIYLPIANVARIMKNAIPQTGKIAKDAKECVQECVSEFISFITSEASERCHQEKRKTINGEDILFAMSTLGFDSYVEPLKLYLQKFREAMKGEKGIGGAVTATDGLSEELTEEAFTNQLPAGLITTDGQQQNVMVYTTSYQQISGVQQIQFS
Specificity
NFYB - nuclear transcription factor Y, beta
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for NFYB Antibody (6H6)
![Western Blot: NFYB Antibody (6H6) [H00004801-M01]](https://resources.bio-techne.com/images/products/NFYB-Antibody-6H6-Western-Blot-H00004801-M01-img0006.jpg "Western Blot: NFYB Antibody (6H6) [H00004801-M01]")
Western Blot: NFYB Antibody (6H6) [H00004801-M01]
Western Blot: NFYB Antibody (6H6) [H00004801-M01] - Analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.Lane 1: NFYB transfected lysate(22.8 KDa).Lane 2: Non-transfected lysate.![Western Blot: NFYB Antibody (6H6) [H00004801-M01]](https://resources.bio-techne.com/images/products/NFYB-Antibody-6H6-Western-Blot-H00004801-M01-img0004.jpg "Western Blot: NFYB Antibody (6H6) [H00004801-M01]")
Western Blot: NFYB Antibody (6H6) [H00004801-M01]
Western Blot: NFYB Antibody (6H6) [H00004801-M01] - Analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi ( Cat # H00004801-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYB monoclonal antibody (M01), clone 6H6 (Cat # H00004801-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: NFYB Antibody (6H6) [H00004801-M01] - Detection limit for recombinant GST tagged NFYB is approximately 0.1ng/ml as a capture antibody.
Applications for NFYB Antibody (6H6)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NFYB
Alternate Names
CAAT box DNA-binding protein subunit B, CBF-A, HAP3CBF-B, NF-YBCCAAT-binding transcription factor subunit A, Nuclear transcription factor Y subunit B, nuclear transcription factor Y subunit beta, nuclear transcription factor Y, beta, Transcription factor NF-Y, B subunit
Entrez Gene IDs
4801 (Human)
Gene Symbol
NFYB
OMIM
189904 (Human)
UniProt
Additional NFYB Products
Product Documents for NFYB Antibody (6H6)
Product Specific Notices for NFYB Antibody (6H6)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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