Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # LHM 2

Concentration

1.0 mg/ml

View all NG2/MCSP Antibodies »

Product Specifications

Immunogen

A 375P cells crude extract

Localization

Cell Membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Scientific Data Images for NG2/MCSP Antibody (LHM 2)

Knockout Validated: NG2/MCSP Antibody (LHM 2) [NB100-2688]

Knockout Validated: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NG2/MCSP (LHM2) knockout (KO) HeLa cell line. PVDF membrane was probed with 1.0 ug/ml of Mouse Anti-Human NG2/MCSP (LHM2) Polyclonal Antibody (Catalog # NB100-2688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for NG2/MCSP (LHM2) at approximately 300 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Western Blot: NG2/MCSP Antibody (LHM 2) [NB100-2688]

Western Blot: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Analysis of NG2 expression in HeLa whole cell lysate.

Immunohistochemistry-Paraffin: NG2/MCSP Antibody (LHM 2) [NB100-2688]

Immunohistochemistry-Paraffin: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Staining of NG2 in human brain using DAB with hematoxylin counterstain.

Flow Cytometry: NG2/MCSP Antibody (LHM 2) [NB100-2688]

Flow Cytometry: NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on SK-MEL-28 cells with NG2/MCSP antibody (LHM 2) NB100-2688AF700 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

Flow Cytometry: NG2/MCSP Antibody (LHM 2) [NB100-2688]

Flow Cytometry: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Antibody was tested at 1:400 in MCF7 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688]

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688PCP (blue) and a matched isotype control NBP2-27287PCP (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex.

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688]

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688]

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A surface stain was performed on A375 Cells with NG2/MCSP (LHM2) antibody NB100-2688 (blue) and a matched isotype control NBP1-43319 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

View 5 more images

Applications for NG2/MCSP Antibody (LHM 2)

Application

Recommended Usage

Flow (Cell Surface)

1 - 2.5 ug/mL

Flow Cytometry

1 ug/ml

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Frozen

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10 - 1:100

Western Blot

1:1000

Application Notes

In WB a band can be seen at ~300 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NG2/MCSP

NG2 (Neuron Glial Antigen 2), also known as MCSP, and CSPG4 is a chondroitin surface proteoglycan. Chondroitin surface proteoglycans play a role in stabilizing cell-substratum interactions during early events of cell spreading. At large, proteoglycans are important for cell proliferation and migration, and may act as signal transducers through C-terminal cytoplasmic binding to scaffolding or signaling proteins. The NG2 glycoprotein is a type I membrane protein expressed in the developing and adult central nervous system (CNS) by subpopulations of glia including oligodendroglial precursor cells (OPCs), and in the developing CNS additionally by pericytes. NG2 is located at the apical cell membrane, however it has also been shown to localize to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Research has found that NG2 may inhibit neuronal growth (PMC1571583), and also acts as a proapoptotic receptor.

Long Name

Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

Alternate Names

AN2, CSPG4, HMW-MAA, MCSP, MCSPG, MEL-CSPG

Entrez Gene IDs

1464 (Human)

Gene Symbol

CSPG4

UniProt

Q6UVK1

Additional NG2/MCSP Products

Product Documents for NG2/MCSP Antibody (LHM 2)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Flow Protocol

Download ICC/IF Protocol

Download Western Blot Protocol

COA

SDS

Download SDS

Product Specific Notices for NG2/MCSP Antibody (LHM 2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

NG2/MCSP Antibody (LHM 2)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2688

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all NG2/MCSP Antibodies »

Product Specifications

Immunogen

Localization

Clonality

Host

Isotype

Scientific Data Images for NG2/MCSP Antibody (LHM 2)

Knockout Validated: NG2/MCSP Antibody (LHM 2) [NB100-2688]

Western Blot: NG2/MCSP Antibody (LHM 2) [NB100-2688]

Immunohistochemistry-Paraffin: NG2/MCSP Antibody (LHM 2) [NB100-2688]

Flow Cytometry: NG2/MCSP Antibody (LHM 2) [NB100-2688]

Flow Cytometry: NG2/MCSP Antibody (LHM 2) [NB100-2688]

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688]

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688]

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688]

Applications for NG2/MCSP Antibody (LHM 2)

Flow (Cell Surface)

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Frozen

Immunohistochemistry-Paraffin

Immunoprecipitation

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: NG2/MCSP

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional NG2/MCSP Products

Product Documents for NG2/MCSP Antibody (LHM 2)

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for NG2/MCSP Antibody (LHM 2)