Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # LHM 2

Format

Azide and BSA Free

Concentration

1 mg/ml

View all NG2/MCSP Antibodies »

Product Specifications

Immunogen

A 375P cells crude extract

Localization

Cell Membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Scientific Data Images for NG2/MCSP Antibody (LHM 2) - Azide and BSA Free

Western Blot: NG2/MCSP Antibody (LHM 2)Azide and BSA Free [NBP2-80873]

Western Blot: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - Analysis of NG2 expression in HeLa whole cell lysate. Image from the standard format of this antibody.

Immunohistochemistry: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Immunohistochemistry: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - Staining of NG2 in human brain using DAB with hematoxylin counterstain. Image from the standard format of this antibody.

Flow Cytometry: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Flow Cytometry: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - Antibody was tested at 1:400 in MCF7 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red). Image from the standard format of this antibody.

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688PCP (blue) and a matched isotype control NBP2-27287PCP (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to PerCP complex.

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - A surface stain was performed on A375 Cells with NG2/MCSP (LHM2) antibody NB100-2688 (blue) and a matched isotype control NBP1-43319 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). Image from the standard format of this antibody.

Flow Cytometry: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Flow Cytometry: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - A cell surface stain was performed on SK-MEL-28 cells with NG2/MCSP antibody (LHM 2) NB100-2688AF700 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 700.

Knockout Validated: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Knockout Validated: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NG2/MCSP (LHM2) knockout (KO) HeLa cell line. PVDF membrane was probed with 1.0 ug/ml of Mouse Anti-Human NG2/MCSP (LHM2) Polyclonal Antibody (Catalog # NB100-2688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for NG2/MCSP (LHM2) at approximately 300 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions. Image from the standard format of this antibody.

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Applications for NG2/MCSP Antibody (LHM 2) - Azide and BSA Free

Application

Recommended Usage

Flow (Cell Surface)

1 - 2.5 ug/ml

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Frozen

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10 - 1:100

Western Blot

1:1000

Application Notes

In WB a band can be seen at ~300 kDa.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NG2/MCSP

NG2 (Neuron Glial Antigen 2), also known as MCSP, and CSPG4 is a chondroitin surface proteoglycan. Chondroitin surface proteoglycans play a role in stabilizing cell-substratum interactions during early events of cell spreading. At large, proteoglycans are important for cell proliferation and migration, and may act as signal transducers through C-terminal cytoplasmic binding to scaffolding or signaling proteins. The NG2 glycoprotein is a type I membrane protein expressed in the developing and adult central nervous system (CNS) by subpopulations of glia including oligodendroglial precursor cells (OPCs), and in the developing CNS additionally by pericytes. NG2 is located at the apical cell membrane, however it has also been shown to localize to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Research has found that NG2 may inhibit neuronal growth (PMC1571583), and also acts as a proapoptotic receptor.

Long Name

Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

Alternate Names

AN2, CSPG4, HMW-MAA, MCSP, MCSPG, MEL-CSPG

Gene Symbol

CSPG4

Additional NG2/MCSP Products

Product Documents for NG2/MCSP Antibody (LHM 2) - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for NG2/MCSP Antibody (LHM 2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

NG2/MCSP Antibody (LHM 2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80873

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all NG2/MCSP Antibodies »

Product Specifications

Immunogen

Localization

Clonality

Host

Isotype

Scientific Data Images for NG2/MCSP Antibody (LHM 2) - Azide and BSA Free

Western Blot: NG2/MCSP Antibody (LHM 2)Azide and BSA Free [NBP2-80873]

Immunohistochemistry: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Flow Cytometry: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Flow Cytometry: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Knockout Validated: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Applications for NG2/MCSP Antibody (LHM 2) - Azide and BSA Free

Flow (Cell Surface)

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Frozen

Immunohistochemistry-Paraffin

Immunoprecipitation

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: NG2/MCSP

Long Name

Alternate Names

Gene Symbol

Additional NG2/MCSP Products

Product Documents for NG2/MCSP Antibody (LHM 2) - Azide and BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for NG2/MCSP Antibody (LHM 2) - Azide and BSA Free