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NGLY1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-29942

Novus Biologicals, part of Bio-Techne
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NBP3-29942

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Between 250 and 300

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NGLY1 Antibody

NGLY1 Antibody

Immunoprecipitation:NGLY1 AntibodyNBP3-29942] -

Immunoprecipitation:NGLY1 AntibodyNBP3-29942] -Detection of human NGLY1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NGLY1 antibody NBP3-29942 (lot NBP3-29942-1) used for IP at 6 µg per reaction. NGLY1 was also immunoprecipitated by rabbit anti-NGLY1 antibody BL21982. For blotting immunoprecipitated NGLY1, NBP3-29942 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for NGLY1 Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NGLY1

NGLY1 is a highly conserved enzyme that catalyzes deglycosylation of misfolded N-linked glycoproteins by cleaving the glycan chain before the proteins are degraded by the proteasome (Zhou et al., 2006).

Alternate Names

EC 3.5.1.52, hPNGase, N-glycanase 1FLJ12409, Peptide:N-glycanase, peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase, PNG1FLJ11005, PNGase

Gene Symbol

NGLY1

Additional NGLY1 Products

Product Documents for NGLY1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NGLY1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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