NHE8 Antibody (7A11)

Novus Biologicals, part of Bio-Techne | Catalog # NB110-62091

Novus Biologicals, part of Bio-Techne
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NB110-62091SS
NB110-62091
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 7A11

Concentration

1.2 mg/ml

View all NHE8 Antibodies »

Product Specifications

Immunogen

A fusion protein containing the C-terminal 89 residues of human NHE8. [Swiss-Prot# Q9Y2E8]

Localization

Golgi apparatus membrane; multi-pass membrane.

Specificity

This is specific for the 85 kDa mature glycosylated form of the protein. It does not recognize the 55 kDa immature form.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NHE8 Antibody (7A11)

Western Blot: NHE8 Antibody (7A11) [NB110-62091]

Western Blot: NHE8 Antibody (7A11) [NB110-62091]

Western Blot: NHE8 Antibody (7A11) [NB110-62091] - Detection of NHE8 (7A11) in HeLa whole cell lysate

Applications for NHE8 Antibody (7A11)

Application
Recommended Usage

Western Blot

2 ug/ml (ECL)
Application Notes
This NHE8 antibody is useful for Western blot analysis where a band is seen at ~80 kDa. Please note that these samples should not be boiled. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

1.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NHE8

NHE (Na+/H+ Exchanger) family members are integral membrane proteins which have been identified to play a key role in catalyzing the extrusion of intracellular proton (H+) ions in exchange for extracellular sodium (Na+) ions for regulation of cellular pH as well as transepithelial ion/water transport. NHE8 is a relatively new member of Na+/H+ exchanger family and is a ubiquitously expressed protein with highest levels detected in epithelial cells of the intestine and the kidney as well as in skeletal muscles. Interestingly, the intestinal/renal expression levels of NHE8 are significantly higher than that of NHE2/NHE3 at a young age, suggesting that NHE8 as an important player for sodium absorption during early development. Localized mainly in Golgi apparatus membranes as multi-pass membrane protein, NHE8 is implicated in regulation of pH to eliminate acids generated by active metabolism or to counter adverse environmental conditions. NHE8 is a major proton extruding system driven by the inward sodium ion chemical gradient and has also been proposed to play role in signaling mechanisms also.

Long Name

Sodium/hydrogen exchanger 8

Alternate Names

KIAA0939, NHE-8, SLC9A8

Gene Symbol

SLC9A8

Additional NHE8 Products

Product Documents for NHE8 Antibody (7A11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NHE8 Antibody (7A11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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