NHERF-2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2450
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-DGSAWKQDPFQ corresponding to internal region according to NP_001123484.1.
Specificity
Please note that this variant has an extra insertion of 11 residues compared to NP_004776.3. The immunizing peptide represents this insertion.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for NHERF-2 Antibody
![Western Blot: NHERF-2 Antibody [NB100-2450]](https://resources.bio-techne.com/images/products/NHERF-2-Antibody-Western-Blot-NB100-2450-img0001.jpg "Western Blot: NHERF-2 Antibody [NB100-2450]")
Western Blot: NHERF-2 Antibody [NB100-2450]
Western Blot: NHERF-2 Antibody [NB100-2450] - (0.3ug/ml) Analysis of human kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: NHERF-2 Antibody [NB100-2450]](https://resources.bio-techne.com/images/products/NHERF-2-Antibody-Immunohistochemistry-Paraffin-NB100-2450-img0002.jpg "Immunohistochemistry-Paraffin: NHERF-2 Antibody [NB100-2450]")
Immunohistochemistry-Paraffin: NHERF-2 Antibody [NB100-2450]
Immunohistochemistry-Paraffin: NHERF-2 Antibody [NB100-2450] - IHC analysis of NHERF-2 in paraffin embedded Human Kidney using NB100-2450 at 3ug/ml. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.Applications for NHERF-2 Antibody
Application
Recommended Usage
Immunohistochemistry
1 - 2 ug/ml
Immunohistochemistry-Paraffin
1 - 2 ug/ml
Peptide ELISA
Detection limit 1:32000
Western Blot
0.3 - 1 ug/ml
Application Notes
WB: Approx. 38 kDa band observed in human kidney lysates (calculated MW of 37.4 kDa band according to BAA33216.1). IHC-P: Human kidney shows strong membranous staining in glomerulus-.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NHERF-2
Alternate Names
E3KARP, Na(+)/H(+) exchange regulatory cofactor NHE-RF2, NHE3RF2, NHERF2MGC104639, NHERF-2NHE3 kinase A regulatory protein E3KARP, OCTS2, SIP-1SRY-interacting protein 1, sodium/hydrogen exchanger, Sodium-hydrogen exchanger regulatory factor 2, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 2, solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2, Solute carrier family 9 isoform A3 regulatory factor 2, TKA-1SIP1, Tyrosine kinase activator protein 1
Entrez Gene IDs
9351 (Human)
Gene Symbol
SLC9A3R2
UniProt
Additional NHERF-2 Products
Product Documents for NHERF-2 Antibody
Product Specific Notices for NHERF-2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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