Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50581
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # AT11G11
Format
BSA Free
Concentration
1 mg/ml
Product Summary for Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free
Immunogen
Recombinant human Nicotinamide N-Methyltransferase/NNMT (1-264aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free
Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11)BSA Free [NBP2-50581]
Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) [NBP2-50581] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: A549 cell lysateImmunocytochemistry/ Immunofluorescence: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free [NBP2-50581]
Immunocytochemistry/Immunofluorescence: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) [NBP2-50581] - ICC/IF analysis of NNMT in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NNMT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free [NBP2-50581]
Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) [NBP2-50581] - Flow cytometry analysis of NNMT in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).Applications for Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free
Application
Recommended Usage
Flow Cytometry
2-5ug
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nicotinamide N-Methyltransferase/NNMT
Long Name
Nicotinamide N-Methyltransferase
Alternate Names
Nicotinamide NMethyltransferase, NNMT
Gene Symbol
NNMT
Additional Nicotinamide N-Methyltransferase/NNMT Products
Product Documents for Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free
Product Specific Notices for Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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