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Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50581

Novus Biologicals, part of Bio-Techne
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NBP2-50581
Conjugate
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # AT11G11

Format

BSA Free

Concentration

1 mg/ml

Product Summary for Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free

Immunogen

Recombinant human Nicotinamide N-Methyltransferase/NNMT (1-264aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11)BSA Free [NBP2-50581]

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11)BSA Free [NBP2-50581]

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) [NBP2-50581] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: A549 cell lysate
Immunocytochemistry/ Immunofluorescence: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free [NBP2-50581]

Immunocytochemistry/ Immunofluorescence: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free [NBP2-50581]

Immunocytochemistry/Immunofluorescence: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) [NBP2-50581] - ICC/IF analysis of NNMT in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NNMT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free [NBP2-50581]

Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free [NBP2-50581]

Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) [NBP2-50581] - Flow cytometry analysis of NNMT in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Applications for Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free

Application
Recommended Usage

Flow Cytometry

2-5ug

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nicotinamide N-Methyltransferase/NNMT

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq]

Long Name

Nicotinamide N-Methyltransferase

Alternate Names

Nicotinamide NMethyltransferase, NNMT

Gene Symbol

NNMT

Additional Nicotinamide N-Methyltransferase/NNMT Products

Product Documents for Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nicotinamide N-Methyltransferase/NNMT Antibody (AT11G11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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