Nicotinamide N-Methyltransferase/NNMT Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03406

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nicotinamide N-Methyltransferase/NNMT Antibodies »

Product Summary for Nicotinamide N-Methyltransferase/NNMT Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human Nicotinamide N-Methyltransferase/NNMT (NP_006160.1). MESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAESQILKHLLKNLFKIFCLDGVKGDLLIDIGSGPTIYQLLSACESFKEIVVTDYSDQNLQELEKWLKKEPEAFDWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLKCDVTQSQPLGAVPLPPADCVLSTLCLDAACPDLPTYCRALRNLGSLLKPGGFLVIMDALKSSYYMIGEQKFSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNEGLFSLVARKLSRPL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nicotinamide N-Methyltransferase/NNMT Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody - Azide and BSA Free [NBP3-03406]

Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody - Azide and BSA Free [NBP3-03406]

Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP3-03406] - Immunohistochemistry of paraffin-embedded human liver using [KO Validated] Nicotinamide N-Methyltransferase/NNMT Rabbit pAb (NBP3-03406) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry: Nicotinamide N-Methyltransferase/NNMT Antibody - Azide and BSA Free [NBP3-03406]

Immunohistochemistry: Nicotinamide N-Methyltransferase/NNMT Antibody - Azide and BSA Free [NBP3-03406]

Immunohistochemistry: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP3-03406] - Immunofluorescence analysis of human liver cancer using [KO Validated] Nicotinamide N-Methyltransferase/NNMT Rabbit pAb (NBP3-03406) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Nicotinamide N-Methyltransferase/NNMT Antibody - Azide and BSA Free

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP3-03406] -

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP3-03406] -analysis of A-549, using NNMT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.

Applications for Nicotinamide N-Methyltransferase/NNMT Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nicotinamide N-Methyltransferase/NNMT

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq]

Long Name

Nicotinamide N-Methyltransferase

Alternate Names

Nicotinamide NMethyltransferase, NNMT

Gene Symbol

NNMT

Additional Nicotinamide N-Methyltransferase/NNMT Products

Product Documents for Nicotinamide N-Methyltransferase/NNMT Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nicotinamide N-Methyltransferase/NNMT Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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