Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81070

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Block/Neutralize, ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rat IgG2b Kappa Clone # mAb 192

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibodies »

Product Specifications

Immunogen

Human muscle AChR.

Specificity

The antibody binds to human muscle AChR with a Kd of 10 pM, to mouse muscle AChR with a Kd of 50 pM, and to rat muscle AChR with a Kd of 646 nM.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Scientific Data Images

Western Blot: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192)Azide and BSA Free [NBP2-81070]

Western Blot: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192)Azide and BSA Free [NBP2-81070]

Western Blot: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192) [NBP2-81070] - Mouse skeletal muscle lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of mAb 192 at 0.03 ug/ml before detection using an anti-mouse IgG1 secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for AChR is 58.9 kDa. Chimeric mouse igG1 version of mAb 192 successfully detected AChR in mouse skeletal muscle tissue lysate. Though this antibody is targetted to the human AChR it also reacts with mouse AChR.
Immunocytochemistry/ Immunofluorescence: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192) - Azide and BSA Free [NBP2-81070]

Immunocytochemistry/ Immunofluorescence: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192) - Azide and BSA Free [NBP2-81070]

Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 1/CHRNA1 Antibody (mAb 192) [NBP2-81070] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of mAb 192 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81070, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.

Applications

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody binds to the human muscle Acetylcholine receptor, which is a ligand-gated ion channel, transducing an action potential into the muscle cells. The antibody binds to the main immunogenic region (MIR) and prevents ligand-binding.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Nicotinic Acetylcholine R alpha 1/CHRNA1

The muscle acetylcholine receptor consiststs of 5 subunits of 4 different types: 2 alpha isoforms and 1 each of beta, gamma, and delta subunits.2 This gene encodes an alpha subunit that plays a role in acetlycholine binding/channel gating. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Long Name

Nicotinic Acetylcholine Receptor alpha 1

Alternate Names

ACHRA, CHRNA1, CMS2A, FCCMS, nAChR alpha 1, nAchRa1, Nicotinic Acetylcholine Ra1, SCCMS

Gene Symbol

CHRNA1

Additional Nicotinic Acetylcholine R alpha 1/CHRNA1 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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