Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00143

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation (Negative), Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HVETRAHAEERLLKK corresponding to internal region according to NP_000735.1.

Epitope

internal region

Specificity

This antibody is expected NOT to cross-react with the similar alpha 2 subunit.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody

Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody [NBP1-00143]

Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody [NBP1-00143]

Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody [NBP1-00143] - Analysis of Nicotinic Acetylcholine Receptor alpha 4 in Rat Brain lysate (35ug protein in RIPA buffer) using this antibody at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

1 - 3 ug/ml
Application Notes
IP: Not suitable. WB: Approx. 70 kDa band observed in rat brain lysates (calculated MW of 70.0 kDa band according to human NP_000735.1and of 70.2 kDa band according to rat NP_077330.1). An additional band of unknown identity was also consistently observed at 22 kDa. This band was successfully blocked by incubation with the immunizing peptide. An anonymous customer has reproted ~75 kDa band in both human and rat tissues as the glycoprotein.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R alpha 4/CHRNA4

This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. [provided by RefSeq]

Long Name

Nicotinic Acetylcholine Receptor alpha 4

Alternate Names

BFNC, CHRNA4, EBN1, ENFL1, nAChR alpha 4, nAchRa4, NACRA4, Nicotinic Acetylcholine Ra4

Entrez Gene IDs

1137 (Human); 11438 (Mouse); 25590 (Rat)

Gene Symbol

CHRNA4

UniProt

NP_000735.1 (Human)

Additional Nicotinic Acetylcholine R alpha 4/CHRNA4 Products

Product Documents for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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