Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52375

Novus Biologicals, part of Bio-Techne
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NBP1-52375
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Key Product Details

Species Reactivity

Validated:

Human, Porcine, Bovine, Canine, Chicken, Equine, Monkey

Cited:

Human

Predicted:

Hamster (94%), Mouse (94%), Opossum (100%), Rabbit (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibodies »

Product Specifications

Immunogen

Peptide with sequence KRPGEDKVRPACQHKQ, from the internal region of the Nicotinic Acetylcholine R alpha 7/CHRNA7 protein sequence according to NP_000737.1.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (88%), Xenopus (88%), Zebrafish (88%), Bat (81%).

Specificity

Human CHRNA7. This antibody also reacts with CHRNA7-FAM7A fusion isoform 1 (NP_647536.1) and isoform 2 (NP_683709.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody [NBP1-52375]

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody [NBP1-52375]

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody [NBP1-52375] - Analysis of anti-CHRNA7 antibody with human hippocampus and isocortex. Image from verified customer review.
Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody [NBP1-52375]

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody [NBP1-52375]

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody [NBP1-52375] - Analysis of anti-CHRNA7 antibody with human brain, cortex at concentration 3 ug/ml.

Applications for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

3-6 ug/ml

Peptide ELISA

1:128000
Application Notes
Use in WB reported in scientific literature (PMID:33850935) IHC: NBP1-52375 was validated for use in IHC on a panel of 21 IHC-P (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity..

Reviewed Applications

Read 1 review rated 5 using NBP1-52375 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

TBS (pH 7.3) and 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R alpha 7/CHRNA7

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediatefast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits.The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conservedtransmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminalextracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeabilityto calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to,alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation thataffects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene islocated in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomallocation involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event inthis region results in a hybrid containing sequence from this gene and a novel FAM7A gene. (provided by RefSeq)

Long Name

Nicotinic Acetylcholine Receptor alpha 7

Alternate Names

CHRNA7, CHRNA7-2, Nicotinic Acetylcholine Ra 7

Entrez Gene IDs

1139 (Human)

Gene Symbol

CHRNA7

UniProt

P36544 (Human)

Additional Nicotinic Acetylcholine R alpha 7/CHRNA7 Products

Product Documents for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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