Nidogen-1/Entactin Antibody (ELM1) [DyLight 650]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08237C
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse, Guinea Pig
Applications
ELISA
Label
DyLight 650 (Excitation = 652 nm, Emission = 672 nm)
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # ELM1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Murine EHS laminin preparation
Reactivity Notes
Does not react with Human.
Localization
Basement membrane
Specificity
It reacts with the intact (150kDa) nidogen as well as its proteolytic fragments of 135kDa, 110kDa, 100kDa, and 80kDa. This monoclonal antibody does not cross-react with other basement membrane components or fibronectin. Entactin/Nidogen is a ubiquitous multi-domain basement membrane protein. Basement membranes are the earliest extracellular matrices produced during embryogenesis. They are synthesized and incorporated into the supramolecular architecture of several components, including laminins, Collagen IV, Nidogen and proteoglycans. Nidogen/Entactin, a sulfated glycoprotein, acts as a link between the extracellular matrix molecules Laminin 1 and Collagen Type IV, and thereby participates in the assembly of basement membranes. Nidogen is synthesized and secreted in primary and established mesenchymal peritubular cells and myoepithelial cells, and it affects adhesion of peritubular cells in an autocrine manner. Nidogen is expressed during embryonic and fetal development exclusively in fully developed basement membranes of the ectoderm and is not expressed in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Applications for Nidogen-1/Entactin Antibody (ELM1) [DyLight 650]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Nidogen-1/Entactin
Alternate Names
Entactin-1, NID1, Nidogen1
Gene Symbol
NID1
Additional Nidogen-1/Entactin Products
Product Documents for Nidogen-1/Entactin Antibody (ELM1) [DyLight 650]
Product Specific Notices for Nidogen-1/Entactin Antibody (ELM1) [DyLight 650]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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