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Key Product Details

Species Reactivity

Mouse, Guinea Pig

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # ELM1

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Murine EHS laminin preparation

Reactivity Notes

Does not react with Human.

Localization

Basement membrane

Specificity

It reacts with the intact (150kDa) nidogen as well as its proteolytic fragments of 135kDa, 110kDa, 100kDa, and 80kDa. This monoclonal antibody does not cross-react with other basement membrane components or fibronectin. Entactin/Nidogen is a ubiquitous multi-domain basement membrane protein. Basement membranes are the earliest extracellular matrices produced during embryogenesis. They are synthesized and incorporated into the supramolecular architecture of several components, including laminins, Collagen IV, Nidogen and proteoglycans. Nidogen/Entactin, a sulfated glycoprotein, acts as a link between the extracellular matrix molecules Laminin 1 and Collagen Type IV, and thereby participates in the assembly of basement membranes. Nidogen is synthesized and secreted in primary and established mesenchymal peritubular cells and myoepithelial cells, and it affects adhesion of peritubular cells in an autocrine manner. Nidogen is expressed during embryonic and fetal development exclusively in fully developed basement membranes of the ectoderm and is not expressed in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07515).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for Nidogen-1/Entactin Antibody (ELM1) - Azide and BSA Free

Application
Recommended Usage

ELISA

2-4 ug/ml
Application Notes
ELISA: Use antibody at 2-4ug/ml. For coating, order antibody without BSA

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Nidogen-1/Entactin

Entactin encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq]

Alternate Names

Entactin-1, NID1, Nidogen1

Gene Symbol

NID1

Additional Nidogen-1/Entactin Products

Product Documents for Nidogen-1/Entactin Antibody (ELM1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nidogen-1/Entactin Antibody (ELM1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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