Nidogen-2 Antibody (4G8)
Novus Biologicals, part of Bio-Techne | Catalog # H00022795-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 4G8
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NID2 (NP_031387.2, 1276 a.a. ~ 1375 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFSIVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK
Specificity
Reacts with nidogen 2 (osteonidogen).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for Nidogen-2 Antibody (4G8)
![Western Blot: Nidogen-2 Antibody (4G8) [H00022795-M01]](https://resources.bio-techne.com/images/products/Nidogen-2-Antibody-4G8-Western-Blot-H00022795-M01-img0004.jpg "Western Blot: Nidogen-2 Antibody (4G8) [H00022795-M01]")
Western Blot: Nidogen-2 Antibody (4G8) [H00022795-M01]
Western Blot: Nidogen-2 Antibody (4G8) [H00022795-M01] - Analysis of NID2 expression in transfected 293T cell line by NID2 monoclonal antibody (M01), clone 4G8.Lane 1: NID2 transfected lysate (Predicted MW: 107.1 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Nidogen-2 Antibody (4G8) [H00022795-M01] - Detection limit for recombinant GST tagged NID2 is 1 ng/ml as a capture antibody.
Applications for Nidogen-2 Antibody (4G8)
Application
Recommended Usage
ELISA
1:100-1:2000
Western Blot
1:100-1:2000
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Nidogen-2
Additional Nidogen-2 Products
Product Documents for Nidogen-2 Antibody (4G8)
Product Specific Notices for Nidogen-2 Antibody (4G8)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...