Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80879
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free [NBP2-80879]](https://resources.bio-techne.com/images/products/Niemann-Pick-C1-Antibody-1318A-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80879-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free [NBP2-80879]")
![Knockout Validated: Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free [NBP2-80879]](https://resources.bio-techne.com/images/products/Niemann-Pick-C1-Antibody-1318A-Azide-and-BSA-Free-Knockout-Validated-NBP2-80879-img0005.jpg "Knockout Validated: Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free [NBP2-80879]")
![Western Blot: Niemann-Pick C1 Antibody (1318A)Azide and BSA Free [NBP2-80879]](https://resources.bio-techne.com/images/products/Niemann-Pick-C1-Antibody-1318A-Azide-and-BSA-Free-Western-Blot-NBP2-80879-img0001.jpg "Western Blot: Niemann-Pick C1 Antibody (1318A)Azide and BSA Free [NBP2-80879]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1318A
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free
Immunogen
A synthetic peptide made to the C-terminal region of human Niemann-Pick C. [UniProt# O15118]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free
Western Blot: Niemann-Pick C1 Antibody (1318A)Azide and BSA Free [NBP2-80879]
Western Blot: Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free [NBP2-80879] - Total protein from human HeLa, Hek293, mouse 3T3 and Rat PC12 cell lines were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free [NBP2-80879]](https://resources.bio-techne.com/images/products/Niemann-Pick-C1-Antibody-1318A-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80879-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free [NBP2-80879]")
Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free [NBP2-80879]
Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free [NBP2-80879] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti- Niemann-Pick C1 Antibody at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved. Image from the standard format of this antibody.![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free [NBP2-80879]](https://resources.bio-techne.com/images/products/Niemann-Pick-C1-Antibody-1318A-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80879-img0004.jpg "Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free [NBP2-80879]")
Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free [NBP2-80879]
Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free [NBP2-80879] - Niemann-Pick Type C1/NPC1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (NBP2-76798) at 3 ug/mL for 1 hour at room temperature followeApplications for Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-5 ug/ml
Immunohistochemistry
5 - 10 ug/ml
Immunohistochemistry-Paraffin
5 - 10 ug/ml
Western Blot
0.5 - 1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Niemann-Pick Type C1/NPC1
Alternate Names
NPC1, SLC65A1
Gene Symbol
NPC1
Additional Niemann-Pick Type C1/NPC1 Products
Product Documents for Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free
Product Specific Notices for Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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