Niemann-Pick type C2 Antibody (012)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90209

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 012

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Niemann-Pick Type C2/NPC2 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Niemann-Pick type C2 (Accession#: NP_006423.1; Met1-Leu151).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Niemann-Pick type C2 Antibody (012)

Western Blot: Niemann-Pick type C2 Antibody (012) [NBP2-90209]

Western Blot: Niemann-Pick type C2 Antibody (012) [NBP2-90209]

Western Blot: Niemann-Pick type C2 Antibody (012) [NBP2-90209] - Anti-NPC2 rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: NIH-3T3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique.
Immunoprecipitation: Niemann-Pick type C2 Antibody (012) [NBP2-90209]

Immunoprecipitation: Niemann-Pick type C2 Antibody (012) [NBP2-90209]

Immunoprecipitation: Niemann-Pick type C2 Antibody (012) [NBP2-90209] - NPC2-His was immunoprecipitated using: Lane A:0.5 mg NIH-3T3 Whole Cell Lysate. 2 uL anti-NPC2-His rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NPC2-His rabbit monoclonal antibody,at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique.

Applications for Niemann-Pick type C2 Antibody (012)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Niemann-Pick Type C2/NPC2

Niemann-Pick type C2 encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy. [provided by RefSeq]

Alternate Names

EDDM1, Epididymal Protein 1, HE1, NiemannPick Type C2, NPC2

Gene Symbol

NPC2

Additional Niemann-Pick Type C2/NPC2 Products

Product Documents for Niemann-Pick type C2 Antibody (012)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Niemann-Pick type C2 Antibody (012)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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