Ninein Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-74631

Novus Biologicals, part of Bio-Techne
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NB100-74631
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Ninein Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1850 and 1900 of human Ninein using the numbering given in entry NP_891991.1 (GeneID 51199).

Marker

Centrosome Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

243 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ninein Antibody

Western Blot: Ninein Antibody [NB100-74631]

Western Blot: Ninein Antibody [NB100-74631]

Western Blot: Ninein Antibody [NB100-74631] - DGAT1 inhibition decreases ncMTOC-related proteins in prostate cancer cells. gamma-tubulin (48 kD), ninein (243 kD), GM130 (140 kD), and CETN1 (20 kD) levels were analyzed by western blot in PC-3 control (CTR) and treated with 1 uM DGAT1 inhibitor or 10 nM PEDF. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-019-39537-z), licensed under a CC-BY license.
Western Blot: Ninein Antibody [NB100-74631]

Western Blot: Ninein Antibody [NB100-74631]

Western Blot: Ninein Antibody [NB100-74631] - Detection of Human and Mouse NINEIN on HeLa whole cell lysate using NB100-74631. NINEIN was also immunoprecipitated by another rabbit anti-NINEIN antibody.

Applications for Ninein Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 1 using NB100-74631 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Ninein

This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.

Alternate Names

Glycogen synthase kinase 3 beta-interacting protein, GSK3B-interacting protein, hNinein, KIAA1565, ninein, ninein (GSK3B interacting protein), ninein centrosomal protein

Entrez Gene IDs

51199 (Human); 18080 (Mouse)

Gene Symbol

NIN

UniProt

Q8N4C6 (Human)

Additional Ninein Products

Product Documents for Ninein Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ninein Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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