NIPBL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93320

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NIPBL Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1025 and 1075 of human Nipped-B-like using the numbering given in entry NP_597677.2 (GeneID 25836).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NIPBL Antibody

Western Blot: NIPBL Antibody [NB100-93320]

Western Blot: NIPBL Antibody [NB100-93320]

Western Blot: NIPBL Antibody [NB100-93320] - Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NIPBL antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunoprecipitation: NIPBL Antibody [NB100-93320]

Immunoprecipitation: NIPBL Antibody [NB100-93320]

Immunoprecipitation: NIPBL Antibody [NB100-93320] - Detection of human NIPBL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NIPBL antibody NB100-93320 (lot 4) used for IP at 3 ug per reaction. NIPBL was also immunoprecipitated by a previous lot of this antibody (lot 3) and rabbit anti-NIPBL antibody NB100-93319. For blotting immunoprecipitated NIPBL, NB100-93320 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for NIPBL Antibody

Application
Recommended Usage

Immunoprecipitation

2-6 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NIPBL

This gene encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and mental retardation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CDLS1, Delangin, DKFZp434L1319, FLJ11203, FLJ12597, FLJ13354, FLJ13648, FLJ44854, IDN3-B, IDN3CDLS, Nipped-B homolog (Drosophila), nipped-B-like protein, Scc2, SCC2 homolog, sister chromatid cohesion 2 homolog

Entrez Gene IDs

25836 (Human)

Gene Symbol

NIPBL

UniProt

NP_597677.2 (Human)

Additional NIPBL Products

Product Documents for NIPBL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NIPBL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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