NIPBL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77974

Novus Biologicals, part of Bio-Techne
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NBP1-77974
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NIPBL Antibodies »

Product Specifications

Immunogen

This affinity-purified NIPBL Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 330-365 of Human NIPBL. (Uniprot: Q6KC79)

Reactivity Notes

A BLAST analysis was used to suggest reactivity with this protein from human, rat, mouse, dog, bovine and orangutan based on 100% homology for the immunogen sequence. Expect reactivity with multiple splice variants of this protein. Cross reactivity with NIPBL homologues from other sources has not been determined.

Specificity

This affinity purified antibody is directed against human NIPBL protein. A BLAST analysis was used to suggest reactivity with this protein from human, rat, mouse, dog, bovine and orangutan based on 100% homology for the immunogen sequence. Expect reactivity with multiple splice variants of this protein. Cross reactivity with NIPBL homologues from other sources has not been determined.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This affinity purified antibody is directed against human NIPBL protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for NIPBL Antibody

Western Blot: NIPBL Antibody [NBP1-77974]

Western Blot: NIPBL Antibody [NBP1-77974]

Western Blot: NIPBL Antibody [NBP1-77974] - Detection of a band ~300 kDa corresponding to IDN3 (arrowhead) in human kidney (lane 1) and human heart (lane 2) whole cell tissue extracts. Approximately 20ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Use HRP conjugated Rb-a-Goat IgG [H&L] MXHu for detection.
Western Blot: NIPBL Antibody [NBP1-77974]

Western Blot: NIPBL Antibody [NBP1-77974]

Western Blot: NIPBL Antibody [NBP1-77974] - Shows detection of bands at ~315 kDa and ~125 kDa corresponding to isoforms of IDN3 (arrow-heads) in mouse heart whole cell tissue extract. Approximately 35 ug of lysate was separated on a 4-8% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Reaction occurred overnight at 4h followed by washes and reaction with a 1:20,000 dilution of IRDye (TM) 800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.
NIPBL Antibody

NIPBL Antibody

Western blot using Affinity Purified anti-IDN3 antibody shows detection of bands at ~315 kDa and ~125 kDa corresponding to isoforms of IDN3 (arrow-heads) in mouse heart whole cell tissue extract

Applications for NIPBL Antibody

Application
Recommended Usage

ELISA

1:2000-1:10000

Western Blot

1:500-1:3000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect bands at 316 kDa in size corresponding to IDN3 by western blotting in the appropriate cell lysate or extract. Splice variants for this protein may show molecular weights of 304 kDa and 122 kDa.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: NIPBL

IDN3 (also known as Colon tumor susceptibility 2, DKFZp434L1319, FLJ13648, Mis 4, Scc-2, Delangin and Sister chromatid cohesion protein) is the mammalian homologue of Scc2 in S. cerevisiae and Mis4 in S. pombe. In yeast, this factor was originally identified genetically as being involved in sister chromatid cohesion; subsequent work has determined that it is not involved in cohesion directly but is required for the loading of cohesins onto chromatin during S phase. It shares 37% sequence identity with Nipped-B gene product of Drosophila, which facilitates enhancer-promoter communication of remote enhancers. The Drosophila protein is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. IDN3 is a nuclear protein that is widely expressed and found at the highest levels in heart, skeletal muscle, fetal and adult liver, and fetal and adult kidney. The protein is also found, albeit at lower levels of expression in thymus, placenta, peripheral leukocyte and small intestine. Multiple isoforms of the protein have been reported.

Alternate Names

CDLS1, Delangin, DKFZp434L1319, FLJ11203, FLJ12597, FLJ13354, FLJ13648, FLJ44854, IDN3-B, IDN3CDLS, Nipped-B homolog (Drosophila), nipped-B-like protein, Scc2, SCC2 homolog, sister chromatid cohesion 2 homolog

Gene Symbol

NIPBL

UniProt

Q6KC79 (Human)

Additional NIPBL Products

Product Documents for NIPBL Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NIPBL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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