NIPP1 Antibody (1G11)

Novus Biologicals, part of Bio-Techne | Catalog # H00005511-M21

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NIPP1 Antibodies »

Product Specifications

Immunogen

PPP1R8 (AAH13360, 1 a.a. ~ 209 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGGEDDELKGLLGLPEEETELDNLTEFNTAHNKRISTLTIEEGNLDIQRPKRMRKNSRVTFSEDDEIINPEDVDPSVGRFRNMVQTAVVPVKKKRVEGPGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSEAGSQPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Reacts with protein phosphatase 1, regulatory (inhibitor) subunit 8.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NIPP1 Antibody (1G11)

Western Blot: NIPP1 Antibody (1G11) [H00005511-M21]

Western Blot: NIPP1 Antibody (1G11) [H00005511-M21]

Western Blot: NIPP1 Antibody (1G11) [H00005511-M21] - Analysis of PPP1R8 expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/ Immunofluorescence: NIPP1 Antibody (1G11) [H00005511-M21]

Immunocytochemistry/ Immunofluorescence: NIPP1 Antibody (1G11) [H00005511-M21]

Immunocytochemistry/Immunofluorescence: NIPP1 Antibody (1G11) [H00005511-M21] - Analysis of monoclonal antibody to PPP1R8 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: NIPP1 Antibody (1G11) [H00005511-M21]

Western Blot: NIPP1 Antibody (1G11) [H00005511-M21]

Western Blot: NIPP1 Antibody (1G11) [H00005511-M21] - Analysis of PPP1R8 expression in PC-12 (Cat # L012V1).
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Applications for NIPP1 Antibody (1G11)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate in WB and recombinant protein in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NIPP1

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq]

Long Name

Nuclear Inhibitor of Protein Phosphatase 1

Alternate Names

ARD1, PPP1R8, PRO2047, RNase E

Entrez Gene IDs

5511 (Human)

Gene Symbol

PPP1R8

UniProt

AAH13360 (Human)

Additional NIPP1 Products

Product Documents for NIPP1 Antibody (1G11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NIPP1 Antibody (1G11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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