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NIPP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1286

Novus Biologicals, part of Bio-Techne
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NB100-1286

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-EAWPGKKPTPSLLI corresponding to C-Terminus according to NP_002704.1, NP_054829.2, NP_612568.1.

Specificity

This antibody is expected to recognise all three human isoforms of this protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NIPP1 Antibody

Western Blot: NIPP1 Antibody [NB100-1286]

Western Blot: NIPP1 Antibody [NB100-1286]

Western Blot: NIPP1 Antibody [NB100-1286] - (1ug/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and NIH3T3 (D) nuclear lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NIPP1 Antibody [NB100-1286]

Immunohistochemistry-Paraffin: NIPP1 Antibody [NB100-1286]

Immunohistochemistry-Paraffin: NIPP1 Antibody [NB100-1286] - 3.8ug/ml staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for NIPP1 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 - 3.8 ug/ml

Immunohistochemistry-Paraffin

2.5 - 3.8 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 2 ug/ml
Application Notes
Western blot: Approx 40kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and NIH3T3 (calculated MW of 38.5kDa according to Human NP_054829.2 and to Mouse NP_666266.1). Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Lung for this particular batch.
IHC: In paraffin embedded Human Thymus shows speckled staining of nuclei.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NIPP1

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.

Long Name

Nuclear Inhibitor of Protein Phosphatase 1

Alternate Names

ARD1, PPP1R8, PRO2047, RNase E

Entrez Gene IDs

5511 (Human); 100336 (Mouse)

Gene Symbol

PPP1R8

Additional NIPP1 Products

Product Documents for NIPP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NIPP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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