Nitrotyrosine Antibody (2A12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30876

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Non-species specific

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2A12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nitrotyrosine Antibodies »

Product Specifications

Immunogen

3-Nitrotyrosine-KLH

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Nitrotyrosine Antibody (2A12)

Nitrotyrosine Antibody (2A12)

Western Blot: Nitrotyrosine Antibody (2A12) [NBP3-30876] -

Western Blot: Nitrotyrosine Antibody (2A12) [NBP3-30876] - A. Western blot using Novus Biologicals' anti 3-nitrotyrosine monoclonal antibody (Cat # NBP3-30876) on 40 ug mouse brain lysate (Lane 1) and 40 ug rat brain lysate (Lane 2). Antibody used at a dilution of 1ug/ml, detected with Supersignal West Pico Substrate-30 second exposure.B. Same experiment blocked with buffer containing 1 mM 3-nitrosine.

Applications for Nitrotyrosine Antibody (2A12)

Application
Recommended Usage

Western Blot

: 1:1000
Application Notes
.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, pH 7.5

Preservative

3mM Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nitrotyrosine

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

Alternate Names

Nitrotyrosine

Additional Nitrotyrosine Products

Product Documents for Nitrotyrosine Antibody (2A12)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Nitrotyrosine Antibody (2A12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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