NK3R/TACR3/Neurokinin B Receptor Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-102

Novus Biologicals, part of Bio-Techne
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NB300-102
NB300-102SS
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat, Guinea Pig, Human (Negative), Sheep

Cited:

Human, Mouse, Rat, Guinea Pig, Ovine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Western Blot (Negative)

Cited:

IF/IHC, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all NK3R/TACR3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide at the C-terminus of rat Neurokinin B Receptor, conjugated to bovine thyroglobulin. [UniProt# P16177]

Reactivity Notes

Rat, mouse and guinea pig in brain, spinal cord and ileum. Customer feedback suggests that human reactivity is negative. Sheep reactivity reported in scientific literature (PMID: 19912479).

Localization

In immunohistochemical experiments staining of the neuronal cell bodies and fibers is seen and staining is believed to be localized to the plasma membrane.

Specificity

NB300-102 is specific to the NK-3 receptor. Specificity was verified by the localization of immunohistochemical staining as well as pre-absorption studies with NK-3 peptide. (1)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for NK3R/TACR3/Neurokinin B Receptor Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry Free-Floating

reported in scientific literature (PMID 25496429)

Immunohistochemistry-Frozen

1:10-1:500. Use reported in scientific literature (PMID 30565563)
Application Notes
It is not useful for Western blot.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NK3R/TACR3

Neurokinins belong to tachykinin family of signalling peptides and the best known human tachykinins are neurokinin B, (encoded by TAC3) and substance P (SP) as well as neurokinin A (NKA), (both encoded by the same TAC1 gene). Neurokinins are widely expressed in CNS, and exert effects on target cells through action at three closely related Gq-coupled neurokinin receptors: NK1R, NK2R, NK3R: encoded in humans by TACR1, TACR2 and TACR3 respectively. None of these neurokinin receptors are entirely specific for SP, NKA or NKB, however, they do show some degree of selectivity, and TACR3/NK3R (neurokinin B receptor or neuromedin-K receptor) behaves as cognate receptor for NKB in vivo. NK3R are predominantly expressed in neurons of PNS/CNS and in particular, in many of forebrain areas, such as frontal, parietal and cingulate cortices, and basal ganglia structures implicated in psychiatric disease states, and can modulate monoaminergic and amino acid neurotransmission within these structures. Neurokinin B and NK3R are also expressed in placenta, kidney, skeletal muscle and lung tissues, and had been implicated in diverse processes, including water homeostasis, pulmonary inflammation, cognition, and preeclampsia.

Long Name

Neurokinin-3 Receptor

Alternate Names

Neurokinin B Receptor, Neuromedin-K Receptor, TAC3RL, TACR3

Entrez Gene IDs

6870 (Human); 24808 (Rat)

Gene Symbol

TACR3

UniProt

P16177 (Rat)

Additional NK3R/TACR3 Products

Product Documents for NK3R/TACR3/Neurokinin B Receptor Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NK3R/TACR3/Neurokinin B Receptor Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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