NKCC1/SLC12A2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52148
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-NALQHSIRLSGVEDH corresponding to internal region according to NP_001037.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for NKCC1/SLC12A2 Antibody
![Immunohistochemistry-Paraffin: NKCC1/SLC12A2 Antibody [NBP1-52148]](https://resources.bio-techne.com/images/products/NKCC1-SLC12A2-Antibody-Immunohistochemistry-Paraffin-NBP1-52148-img0002.jpg "Immunohistochemistry-Paraffin: NKCC1/SLC12A2 Antibody [NBP1-52148]")
Immunohistochemistry-Paraffin: NKCC1/SLC12A2 Antibody [NBP1-52148]
Immunohistochemistry-Paraffin: NKCC1/SLC12A2 Antibody [NBP1-52148] - Staining of Human Colon. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.![Flow Cytometry: NKCC1/SLC12A2 Antibody [NBP1-52148]](https://resources.bio-techne.com/images/products/NKCC1-SLC12A2-Antibody-Flow-Cytometry-NBP1-52148-img0004.jpg "Flow Cytometry: NKCC1/SLC12A2 Antibody [NBP1-52148]")
Flow Cytometry: NKCC1/SLC12A2 Antibody [NBP1-52148]
Flow Cytometry: NKCC1/SLC12A2 Antibody [NBP1-52148] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.![Immunofluorescence: NKCC1/SLC12A2 Antibody [NBP1-52148]](https://resources.bio-techne.com/images/products/NKCC1-SLC12A2-Antibody-Immunofluorescence-NBP1-52148-img0003.jpg "Immunofluorescence: NKCC1/SLC12A2 Antibody [NBP1-52148]")
Immunofluorescence: NKCC1/SLC12A2 Antibody [NBP1-52148]
Immunofluorescence: NKCC1/SLC12A2 Antibody [NBP1-52148] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic/vesicle and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Applications for NKCC1/SLC12A2 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/mL
Immunohistochemistry
3.75 ug/mL
Immunohistochemistry-Paraffin
3.75 ug/mL
Peptide ELISA
Detection limit 1:64000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NKCC1/SLC12A2
Long Name
Sodium/Potassium/Chloride Transporter NKCC1
Alternate Names
BSC2, SLC12A2
Gene Symbol
SLC12A2
UniProt
Additional NKCC1/SLC12A2 Products
Product Documents for NKCC1/SLC12A2 Antibody
Product Specific Notices for NKCC1/SLC12A2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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