NKX2.5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51953

Novus Biologicals, part of Bio-Techne
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NBP1-51953
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all NKX2.5 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PRAYSDPDPAKDPR, from the internal region of the protein sequence according to NP_004378.1; NP_001159647.1; NP_001159648.1.

Epitope

C-PRAYSDPDPAKDPR

Specificity

This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).

Marker

Cardiac Progenitor Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NKX2.5 Antibody

Western Blot: NKX2.5 Antibody [NBP1-51953]

Western Blot: NKX2.5 Antibody [NBP1-51953]

Western Blot: NKX2.5 Antibody [NBP1-51953] - (1ug/ml) staining of Human (A), Mouse (B) and (2ug/ml) Rat (C) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
NKX2.5 Antibody

Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP1-51953] -

Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP1-51953] - Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.
NKX2.5 Antibody

Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP1-51953] -

Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP1-51953] - (6ug/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for NKX2.5 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5-6 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

1-3 ug/mL
Application Notes
WB: Approx. 38kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 34.9kDa according to Human NP_004378.1, 34.2kDa according to Mouse NP_032726.1 and 34.3kDa according to Rat NP_446103.2). Primary incubation 1 hour at room temperature.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NKX2.5

Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM]

Long Name

NK2 Homeobox 5

Alternate Names

CSX1, NKX2-5, NKX2E

Entrez Gene IDs

1482 (Human); 18091 (Mouse); 114109 (Rat)

Gene Symbol

NKX2-5

UniProt

NP_004378.1 (Human)

Additional NKX2.5 Products

Product Documents for NKX2.5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NKX2.5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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