NKX3.1 Antibody (NKX3.1/2576)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79736

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # NKX3.1/2576

Concentration

0.2 mg/ml

View all NKX3.1 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 92-224) of human NKX3.1 protein (exact sequence is proprietary) (Uniprot: Q99801)

Localization

Nuclear

Marker

Metastatic Prostate Adenocarcinoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79871)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for NKX3.1 Antibody (NKX3.1/2576)

Immunohistochemistry-Paraffin: NKX3.1 Antibody (NKX3.1/2576) [NBP2-79736]

Immunohistochemistry-Paraffin: NKX3.1 Antibody (NKX3.1/2576) [NBP2-79736]

Immunohistochemistry-Paraffin: NKX3.1 Antibody (NKX3.1/2576) [NBP2-79736] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with NKX3.1 Antibody (NKX3.1/2576).
Immunohistochemistry-Paraffin: NKX3.1 Antibody (NKX3.1/2576) [NBP2-79736]

Immunohistochemistry-Paraffin: NKX3.1 Antibody (NKX3.1/2576) [NBP2-79736]

Immunohistochemistry-Paraffin: NKX3.1 Antibody (NKX3.1/2576) [NBP2-79736] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with NKX3.1 Antibody (NKX3.1/2576).
Protein Array: NKX3.1 Antibody (NKX3.1/2576) [NBP2-79736]

Protein Array: NKX3.1 Antibody (NKX3.1/2576) [NBP2-79736]

Protein Array: NKX3.1 Antibody (NKX3.1/2576) [NBP2-79736] - Analysis of Protein Array containing more than 19,000 full-length human proteins using NKX3.1 Antibody (NKX3.1/2576). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for NKX3.1 Antibody (NKX3.1/2576)

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: NKX3.1

Nkx3.1 is a transcription factor that may play an important role in regulating proliferation of glandular epithelium and in the formation of ducts in the prostate. It has been thought to be one of the target genes of the 8p21 loss of heterozygosity, common in prostate cancer. But neither disruption of the coding region of the gene, nor mutations have been found in prostate cancer.

Long Name

NK3 Homeobox 1

Alternate Names

BAPX2, NKX3-1, NKX3A

Gene Symbol

NKX3-1

UniProt

Q99801 (Human)

Additional NKX3.1 Products

Product Documents for NKX3.1 Antibody (NKX3.1/2576)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NKX3.1 Antibody (NKX3.1/2576)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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