NKX3.1 Antibody (NKX3.1/2576) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79871
![Immunohistochemistry-Paraffin: NKX3.1 Antibody (NKX3.1/2576) - Azide and BSA Free [NBP2-79871]](https://resources.bio-techne.com/images/products/NKX3-1-Antibody-NKX3-1-2576-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79871-img0002.jpg "Immunohistochemistry-Paraffin: NKX3.1 Antibody (NKX3.1/2576) - Azide and BSA Free [NBP2-79871]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2c Kappa Clone # NKX3.1/2576
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 92-224) of human NKX3.1 protein (exact sequence is proprietary) (Uniprot: Q99801)
Localization
Nuclear
Marker
Metastatic Prostate Adenocarcinoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2c Kappa
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79736).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for NKX3.1 Antibody (NKX3.1/2576) - Azide and BSA Free
Immunohistochemistry-Paraffin: NKX3.1 Antibody (NKX3.1/2576) - Azide and BSA Free [NBP2-79871]
Immunohistochemistry-Paraffin: NKX3.1 Antibody (NKX3.1/2576) - Azide and BSA Free [NBP2-79871] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with NKX3.1-Monospecific Mouse Monoclonal Antibody (NKX3.1/2576).![Immunohistochemistry-Paraffin: NKX3.1 Antibody (NKX3.1/2576) - Azide and BSA Free [NBP2-79871]](https://resources.bio-techne.com/images/products/NKX3-1-Antibody-NKX3-1-2576-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79871-img0001.jpg "Immunohistochemistry-Paraffin: NKX3.1 Antibody (NKX3.1/2576) - Azide and BSA Free [NBP2-79871]")
Immunohistochemistry-Paraffin: NKX3.1 Antibody (NKX3.1/2576) - Azide and BSA Free [NBP2-79871]
Immunohistochemistry-Paraffin: NKX3.1 Antibody (NKX3.1/2576) - Azide and BSA Free [NBP2-79871] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with NKX3.1-Monospecific Mouse Monoclonal Antibody (NKX3.1/2576).![Protein Array: NKX3.1 Antibody (NKX3.1/2576) - Azide and BSA Free [NBP2-79871]](https://resources.bio-techne.com/images/products/NKX3-1-Antibody-NKX3-1-2576-Azide-and-BSA-Free-Protein-Array-NBP2-79871-img0003.jpg "Protein Array: NKX3.1 Antibody (NKX3.1/2576) - Azide and BSA Free [NBP2-79871]")
Protein Array: NKX3.1 Antibody (NKX3.1/2576) - Azide and BSA Free [NBP2-79871]
Protein Array: NKX3.1 Antibody (NKX3.1/2576) - Azide and BSA Free [NBP2-79871] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.Applications for NKX3.1 Antibody (NKX3.1/2576) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1 - 2 ug/mL
Immunohistochemistry-Paraffin
1 - 2 ug/mL
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: NKX3.1
Long Name
NK3 Homeobox 1
Alternate Names
BAPX2, NKX3-1, NKX3A
Gene Symbol
NKX3-1
Additional NKX3.1 Products
Product Documents for NKX3.1 Antibody (NKX3.1/2576) - Azide and BSA Free
Product Specific Notices for NKX3.1 Antibody (NKX3.1/2576) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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