NKX6.1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82553

Novus Biologicals, part of Bio-Techne
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NBP1-82553
NBP1-82553-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NKX6.1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PLGTHNPGGLKPPATGGLSSLGSPPQQLSAATPHGINDILSRPSM

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:31872528).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NKX6.1 Antibody

Immunocytochemistry/ Immunofluorescence: NKX6.1 Antibody [NBP1-82553]

Immunocytochemistry/ Immunofluorescence: NKX6.1 Antibody [NBP1-82553]

Immunocytochemistry/Immunofluorescence: NKX6.1 Antibody [NBP1-82553] - Comparison of the hiPSC differentiation outcome according to the stage of encapsulation. Whole mount immunofluorescence of encapsulated cells stained for insulin (green), NKX6.1 (red), PDX1 (purple) and DAPI (blue), gamma correction 0.4. Scale bars: 10 um. Graphs data are shown as mean +/- SEM. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-019-57305-x), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553]

Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553]

Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553] - Staining of human colon shows no positivity in glandular cells as expected.
Immunocytochemistry/ Immunofluorescence: NKX6.1 Antibody [NBP1-82553]

Immunocytochemistry/ Immunofluorescence: NKX6.1 Antibody [NBP1-82553]

Immunocytochemistry/Immunofluorescence: NKX6.1 Antibody [NBP1-82553] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
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Applications for NKX6.1 Antibody

Application
Recommended Usage

Flow Cytometry

Reported in scientific literature (PMID: 30930126).

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NKX6.1

In the pancreas, NKX6.1 is required for the development of beta cells and is a potent bifunctional transcription regulator that binds to AT-rich sequences within the promoter region of target genes Iype et al. (2004) [PubMed 15056733].[supplied by OMIM]

Long Name

NK6 Homeobox 1

Alternate Names

NKX6-1, NKX6A

Entrez Gene IDs

4825 (Human)

Gene Symbol

NKX6-1

UniProt

P78426 (Human)

Additional NKX6.1 Products

Product Documents for NKX6.1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NKX6.1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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