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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG1 Clone # Eowyn-1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant mouse NLRC3 (card domain).

Specificity

Detects overexpressed mouse NLRC3. Doest not detect human NLRC3. Although NLRC3 (mouse), mAb (Eowyn-1) is strong and specific (see figure), the endogenous protein has not been detected yet with this antibody (using NLRC3 KO versus WT immune cells). NLRC3 protein is probably expressed at very low levels or its translation / stability is triggered by stimuli still to be discovered .

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for NLRC3 Antibody (Eowyn-1) [CoraFluor™ 1]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: NLRC3

Alternate Names

CARD15-Like Protein, Caterpiller Protein 16.2, CLR16.2, Leucine Rich Repeat And CARD Domain Containing 3, NLR Family, CARD Domain Containing 3, NOD3, NOD-Like Receptor C3, Nucleotide-Binding Oligomerization Domain Protein 3, Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And CARD Domain Containing 3, Protein NLRC3

Gene Symbol

NLRC3

Additional NLRC3 Products

Product Documents for NLRC3 Antibody (Eowyn-1) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NLRC3 Antibody (Eowyn-1) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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