NLRP1/NALP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56147

Novus Biologicals, part of Bio-Techne
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NB100-56147
NB100-56147SS
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all NLRP1/NALP1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 1058-1077 (CVPSPASQGDLHTKPLGTDD) of human NALP1 (NAC/CARD7) was used as immunogen, GenBank no. NP_127497.1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NLRP1/NALP1 Antibody

Immunohistochemistry-Paraffin: NLRP1/NALP1 Antibody [NB100-56147]

Immunohistochemistry-Paraffin: NLRP1/NALP1 Antibody [NB100-56147]

Immunohistochemistry-Paraffin: NLRP1/NALP1 Antibody [NB100-56147] - FFPE normal human bone marrow stained for NALP1 (CARD7/NAP) using NB100-56147 at 1:2000. Hematoxylin-eosin counterstain. Staining is seen in differentiated granulocytes.
Immunoprecipitation: NLRP1/NALP1 Antibody [NB100-56147]

Immunoprecipitation: NLRP1/NALP1 Antibody [NB100-56147]

Immunoprecipitation: NLRP1/NALP1 Antibody [NB100-56147] - Analysis of NLRP1/NALP1(NAC/CARD7) in Jurkat cells using NB100-56148 and NB100-56147 NALP1 antibodies. Cell lysates were immunoprecipitated with NB100-56148 (2 uL/million cells) and Western blotted with NB100-56147 at 1:2000.

Applications for NLRP1/NALP1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

1:50 - 1:200

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NLRP1/NALP1

NALP1 (also known as NAC/CARD7) is a member of the CATERPILLER (CLR) diverse multigene superfamily of immune regulatory proteins (Ting and Davis, 2005). CLR proteins have a variable N-terminal domain, followed by a nucleotide-binding domain (NBD) and leucine-rich repeats (LRR). The N-terminal domain consists of transactivation, CARD, Pyrin or BIR domains, or in some cases is undefined. The CLR family, also known as the NOD family, has its ancient roots in the plant kingdom and is related to the disease resistance (R) gene family that mediates plant immune responses. CLR proteins participate in both innate and adaptive immunity in mammals, some act as sensors that detect pathogen products, and several CLR genes have been linked to susceptibility to immunological diseases. It is thought that the CLR family may have a fundamental importance for the function of the mammalian innate/ancient immune system on par with the Toll-like receptor (TLR) family. NALP1, a member of NALP CLR subfamily, has an N-terminal pyrin domain (PYD), followed by an NBD, LRR, and a C-terminal CARD domain (reviewed in Tschopp. NALP1 is a component of both the inflammasome and apoptosome, suggesting that NALP1 it has important roles in both inflammation and apoptosis. This antibody recognizes NALP1; human NALP1 is a 1473 amino acid protein and migrates at approx. 166 kDa on SDS-PAGE. However, multiple splice variants encoding distinct NALP1 isoforms with varying amino acid lengths have been described and thus the molecular weight detected may vary depending on the isoform(s) expressed.

Long Name

NLR Family, Pyrin Domain Containing 1

Alternate Names

CARD7, CLR17.1, DEFCAP, NALP1, PP1044, SLEV1, VAMAS1

Entrez Gene IDs

22861 (Human)

Gene Symbol

NLRP1

Additional NLRP1/NALP1 Products

Product Documents for NLRP1/NALP1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NLRP1/NALP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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