NM23-H1 Antibody (4B2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47398

Novus Biologicals, part of Bio-Techne
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NBP1-47398
NBP1-47398-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4B2

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all NM23-H1 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human NM23-H1 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NM23-H1 Antibody (4B2) - BSA Free

Western Blot: NM23-H1 Antibody (4B2)BSA Free [NBP1-47398]

Western Blot: NM23-H1 Antibody (4B2)BSA Free [NBP1-47398]

Western Blot: NM23-H1 Antibody (4B2) [NBP1-47398] - Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (4B2) - BSA Free [NBP1-47398]

Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (4B2) - BSA Free [NBP1-47398]

Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (4B2) [NBP1-47398] - Immunofluorescence analysis of Hela cells using NME1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: NM23-H1 Antibody (4B2) - BSA Free [NBP1-47398]

Immunohistochemistry-Paraffin: NM23-H1 Antibody (4B2) - BSA Free [NBP1-47398]

Immunohistochemistry-Paraffin: NM23-H1 Antibody (4B2) [NBP1-47398] - Immunohistochemical analysis of paraffin-embedded human Placenta tissues using anti-NME1 mouse mAb.
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Applications for NM23-H1 Antibody (4B2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NM23-H1

The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and -H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nucleasehypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

Long Name

Non-metastatic Protein 23 Homolog 1

Alternate Names

GAAD, NDKA, NDPKA, NM23A, NM23H1, NME1

Gene Symbol

NME1

Additional NM23-H1 Products

Product Documents for NM23-H1 Antibody (4B2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NM23-H1 Antibody (4B2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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