NM23-H1 Antibody (OTI4H2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46186

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI4H2

Concentration

1 mg/ml

View all NM23-H1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NME1 (NP_937818) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

19.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NM23-H1 Antibody (OTI4H2)

Western Blot: NM23-H1 Antibody (OTI4H2) [NBP2-46186]

Western Blot: NM23-H1 Antibody (OTI4H2) [NBP2-46186]

Western Blot: NM23-H1 Antibody (4H2) [NBP2-46186] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NM23-H1.
Immunohistochemistry: NM23-H1 Antibody (OTI4H2) [NBP2-46186]

Immunohistochemistry: NM23-H1 Antibody (OTI4H2) [NBP2-46186]

Immunohistochemistry: NM23-H1 Antibody (4H2) [NBP2-46186] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: NM23-H1 Antibody (OTI4H2) [NBP2-46186]

Immunohistochemistry: NM23-H1 Antibody (OTI4H2) [NBP2-46186]

Immunohistochemistry: NM23-H1 Antibody (4H2) [NBP2-46186] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for NM23-H1 Antibody (OTI4H2)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NM23-H1

The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and -H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nucleasehypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

Long Name

Non-metastatic Protein 23 Homolog 1

Alternate Names

GAAD, NDKA, NDPKA, NM23A, NM23H1, NME1

Gene Symbol

NME1

Additional NM23-H1 Products

Product Documents for NM23-H1 Antibody (OTI4H2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NM23-H1 Antibody (OTI4H2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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