NM23-H1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19549

Novus Biologicals, part of Bio-Techne
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NBP2-19549
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (94%), Canine (92%), Chimpanzee (100%), Guinea Pig (92%), Porcine (95%), Rabbit (92%), Rat (95%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NM23-H1 Antibodies »

Product Summary for NM23-H1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human nm23-H1. The exact sequence is proprietary.

Reactivity Notes

Chicken (84%).

Localization

Cytoplasm; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NM23-H1 Antibody

Western Blot: NM23-H1 Antibody [NBP2-19549]

Western Blot: NM23-H1 Antibody [NBP2-19549]

Western Blot: NM23-H1 Antibody [NBP2-19549] - Wild-type (WT) and nm23-H1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody [NBP2-19549]

Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody [NBP2-19549]

Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody [NBP2-19549] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:100 dilution.
Western Blot: NM23-H1 Antibody [NBP2-19549]

Western Blot: NM23-H1 Antibody [NBP2-19549]

Western Blot: NM23-H1 Antibody [NBP2-19549] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 12% SDS PAGE gel, diluted at 1:1000.
View 1 more images

Applications for NM23-H1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NM23-H1

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq]

Long Name

Non-metastatic Protein 23 Homolog 1

Alternate Names

GAAD, NDKA, NDPKA, NM23A, NM23H1, NME1

Gene Symbol

NME1

UniProt

P15531

Additional NM23-H1 Products

Product Documents for NM23-H1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NM23-H1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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