Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S308-48

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NMDAR1 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 42-361 (extracellular N-terminus) of rat NR1

Localization

Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane

Specificity

Detects 105kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for NMDAR1 Antibody (S308-48)

Western Blot: NMDAR1 Antibody (S308-48) [NBP2-59327]

Western Blot: NMDAR1 Antibody (S308-48) [NBP2-59327]

Western Blot: NMDAR1 Antibody (S308-48) [NBP2-59327] - Western Blot analysis of Rat brain membrane lysate showing detection of NMDAR1 NMDA receptor protein using Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody, Clone S308-48 (NBP2-59327). Primary Antibody: Mouse Anti-NMDAR1 NMDA receptor Monoclonal Antibody (NBP2-59327) at 1:1000.
Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (S308-48) [NBP2-59327]

Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (S308-48) [NBP2-59327]

Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (S308-48) [NBP2-59327] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NMDAR1/NR1 Monoclonal Antibody, Clone S308-48 (NBP2-59327). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-NMDAR1/NR1 Monoclonal Antibody (NBP2-59327) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) NMDAR1/NR1 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (S308-48) [NBP2-59327]

Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (S308-48) [NBP2-59327]

Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (S308-48) [NBP2-59327] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN1/NR1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GluN1/NR1 Antibody (D) Composite.

Applications for NMDAR1 Antibody (S308-48)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: NMDAR1

N-methyl-D-aspartate (NMDA) receptors are ligand-gated ion channels that have a high permeability to calcium found in the central nervous system. The receptor consists of a number of distinct ligand binding domains, and the presence of both glutamate and glycine are required for full activation of the channel. Within the channel there is also a binding site for magnesium, which, when occupied, propagates a voltage-dependent channel block. Other binding sites are also found in the receptor, including a zinc-binding site and an inter-channel site that binds specific channel blockers such as phencyclidine (PCP) and related compounds. The NMDA receptor has been demonstrated to play an essential role in long-term potentiation (LTP), a phenomenon that has been implicated to be the basis for learning and memory. The influx of calcium as a result of channel activation is thought to be responsible for neuronal plasticity and glutamate neurotoxicity. A number of different NMDA receptor subunits have been cloned that may possess different functional and localization properties. The NMDA-R1 subunit (NR1) is expressed throughout the brain, while the NMDA-R2 subunits (NR2A, NR2B, NR2C, and NR2D) have a more specific localization pattern. The NMDA receptor subunits differ also in glycine sensitivity, the relative strength of the magnesium channel block, and their respective agonist-dependent deactivation time.

Alternate Names

glutamate [NMDA] receptor subunit zeta-1, glutamate receptor, ionotropic, N-methyl D-aspartate 1, NMDA1, NMDAR1glutamate [NMDA] receptor subunit zeta 1, NMD-R1, N-methyl-D-aspartate receptor channel, subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NR1

Gene Symbol

GRIN1

Additional NMDAR1 Products

Product Documents for NMDAR1 Antibody (S308-48)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NMDAR1 Antibody (S308-48)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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