NMDAR1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13158

Novus Biologicals, part of Bio-Techne
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NBP3-13158
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat, Canine

Predicted:

Human (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Tumorsphere

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NMDAR1 Antibodies »

Product Summary for NMDAR1 Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse NMDAR1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for NMDAR1 Antibody

Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody [NBP3-13158]

Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody [NBP3-13158]

Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody [NBP3-13158] - NMDAR1 antibody detects NMDAR1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NMDAR1 stained by NMDAR1 antibody (NBP3-13158) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: NMDAR1 Antibody [NBP3-13158]

Immunohistochemistry-Frozen: NMDAR1 Antibody [NBP3-13158]

Immunohistochemistry-Frozen: NMDAR1 Antibody [NBP3-13158] - NMDAR1 antibody detects NMDAR1 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: NMDAR1 protein stained by NMDAR1 antibody (NBP3-13158) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:250. Blue: Fluoroshield with DAPI.
Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody [NBP3-13158]

Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody [NBP3-13158]

Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody [NBP3-13158] - NMDAR1 antibody detects NMDAR1 protein at synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: NMDAR1 protein stained by NMDAR1 antibody (NBP3-13158) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue:Fluoroshield with DAPI.
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Applications for NMDAR1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Tumorsphere

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NMDAR1

N-methyl-D-aspartate (NMDA) receptors are ligand-gated ion channels that have a high permeability to calcium found in the central nervous system. The receptor consists of a number of distinct ligand binding domains, and the presence of both glutamate and glycine are required for full activation of the channel. Within the channel there is also a binding site for magnesium, which, when occupied, propagates a voltage-dependent channel block. Other binding sites are also found in the receptor, including a zinc-binding site and an inter-channel site that binds specific channel blockers such as phencyclidine (PCP) and related compounds. The NMDA receptor has been demonstrated to play an essential role in long-term potentiation (LTP), a phenomenon that has been implicated to be the basis for learning and memory. The influx of calcium as a result of channel activation is thought to be responsible for neuronal plasticity and glutamate neurotoxicity. A number of different NMDA receptor subunits have been cloned that may possess different functional and localization properties. The NMDA-R1 subunit (NR1) is expressed throughout the brain, while the NMDA-R2 subunits (NR2A, NR2B, NR2C, and NR2D) have a more specific localization pattern. The NMDA receptor subunits differ also in glycine sensitivity, the relative strength of the magnesium channel block, and their respective agonist-dependent deactivation time.

Alternate Names

glutamate [NMDA] receptor subunit zeta-1, glutamate receptor, ionotropic, N-methyl D-aspartate 1, NMDA1, NMDAR1glutamate [NMDA] receptor subunit zeta 1, NMD-R1, N-methyl-D-aspartate receptor channel, subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NR1

Gene Symbol

GRIN1

Additional NMDAR1 Products

Product Documents for NMDAR1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NMDAR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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