NMDAR2B [p Ser1480] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-61103
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse
Predicted:
Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Primate (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1480 conjugated to KLH. Accession # Q00960
Modification
p Ser1480
Specificity
Specific for endogenous levels of the ~180 kDa NMDAR2B protein phosphorylated at Ser1480. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NMDAR2B [p Ser1480] Antibody
Western Blot: NMDAR2B [p Ser1480] Antibody [NB100-61103]
Western Blot: NMDAR2B [p Ser1480] Antibody [NB100-61103] - Changes of NMDAR2B phosphorylation levels in the hippocampus and prefrontal cortex of P28 mice after earlylife propofol exposure. Typical results of western blot showed the changes in pSer1480 NMDAR2B phosphorylation levels in cytosol and particulate fractions of the hippocampus and prefrontal cortex of P28 mice after earlylife propofol exposure. D6 mice group treated with propofol (100 mg/kg body weight, i.p.) daily P6-P11; D1 mice group treated with propofil (100 mg/kg, i.p.) at P6 and then normal saline (10 mL/kg, i.p.) from P7-P11; N mice group received normal saline (10 mL/kg, i.p.) from P6-P11. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28729940/) licensed under a CC-BY license.Western Blot: NMDAR2B [p Ser1480] Antibody [NB100-61103]
Western Blot: NMDAR2B [p Ser1480] Antibody [NB100-61103] - Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Ser1480 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser1480 NMDA NR2B subunit antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.Applications for NMDAR2B [p Ser1480] Antibody
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NMDAR2B
Alternate Names
glutamate [NMDA] receptor subunit epsilon-2, glutamate receptor subunit epsilon-2, glutamate receptor, ionotropic, N-methyl D-aspartate 2B, hNR3, MGC142180, NMDAR2BMGC142178, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3
Entrez Gene IDs
24410 (Rat)
Gene Symbol
GRIN2B
UniProt
Additional NMDAR2B Products
Product Documents for NMDAR2B [p Ser1480] Antibody
Product Specific Notices for NMDAR2B [p Ser1480] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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