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NMDAR2B [p Ser1480] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61103

Novus Biologicals, part of Bio-Techne
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NB100-61103

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Primate (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1480 conjugated to KLH. Accession # Q00960

Modification

p Ser1480

Specificity

Specific for endogenous levels of the ~180 kDa NMDAR2B protein phosphorylated at Ser1480. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NMDAR2B [p Ser1480] Antibody

Western Blot: NMDAR2B [p Ser1480] Antibody [NB100-61103]

Western Blot: NMDAR2B [p Ser1480] Antibody [NB100-61103]

Western Blot: NMDAR2B [p Ser1480] Antibody [NB100-61103] - Changes of NMDAR2B phosphorylation levels in the hippocampus and prefrontal cortex of P28 mice after earlylife propofol exposure. Typical results of western blot showed the changes in pSer1480 NMDAR2B phosphorylation levels in cytosol and particulate fractions of the hippocampus and prefrontal cortex of P28 mice after earlylife propofol exposure. D6 mice group treated with propofol (100 mg/kg body weight, i.p.) daily P6-P11; D1 mice group treated with propofil (100 mg/kg, i.p.) at P6 and then normal saline (10 mL/kg, i.p.) from P7-P11; N mice group received normal saline (10 mL/kg, i.p.) from P6-P11. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28729940/) licensed under a CC-BY license.
Western Blot: NMDAR2B [p Ser1480] Antibody [NB100-61103]

Western Blot: NMDAR2B [p Ser1480] Antibody [NB100-61103]

Western Blot: NMDAR2B [p Ser1480] Antibody [NB100-61103] - Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Ser1480 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser1480 NMDA NR2B subunit antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Applications for NMDAR2B [p Ser1480] Antibody

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NMDAR2B

The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). It has been shown that phosphorylation of Ser1480 disrupts the interaction of NR2B with the PDZ domains of PSD-95 and SAP102 and decreases surface NR2B expression in neurons (Chung et al., 2004).

Alternate Names

glutamate [NMDA] receptor subunit epsilon-2, glutamate receptor subunit epsilon-2, glutamate receptor, ionotropic, N-methyl D-aspartate 2B, hNR3, MGC142180, NMDAR2BMGC142178, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3

Entrez Gene IDs

24410 (Rat)

Gene Symbol

GRIN2B

UniProt

Additional NMDAR2B Products

Product Documents for NMDAR2B [p Ser1480] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NMDAR2B [p Ser1480] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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