NOD2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27328
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
A synthetic peptide corresponding to amino acids 582-610 of human NOD2/CARD15 was used as immunogen; GenBank no. NP_071445 NOD2 is a 1040 amino acid protein. The immunogen sequence is 100% conserved in certain NOD2 isoforms and mutations. The immunogen sequence corresponds to amino acids 45-63 of a short form of NOD2. This NOD2 short form is 273 amino acids; GenBank no.AAk70868.1 aa 582-600; N-Terminus; human
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 20615568).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NOD2 Antibody
Western Blot: NOD2 Antibody [NBP2-27328]
Western Blot: NOD2 Antibody [NBP2-27328] - Analysis of NOD2 using this antibody at 1:2000. 30 ug total protein was loaded per lane. Samples from human tumor cell lines: PPC-1 (prostate carcinoma), NCI-H226 and NCI-H332M (lung carcinoma), SF-268 (glioblastoma), UO-31 and A498 (renal/kidney carcinoma), SK-MEL-5 and SK-MEL-28 (melanoma), and MCF-7 (breast carcinoma). Lymphoma is from a cell line generated from a tumor growing in nude mice following injection of primary human lymphoma cells.Immunohistochemistry-Paraffin: NOD2 Antibody [NBP2-27328]
Immunohistochemistry-Paraffin: NOD2 Antibody [NBP2-27328] - Formalin-fixed, paraffin-embedded normal human colon stained for NOD2 expression using this antibody at 1:2000. A, tissue core from a colon tissue microarray. A1, high magnification of A. Hematoxylin-eosin counterstain.Applications for NOD2 Antibody
Application
Recommended Usage
Immunohistochemistry-Frozen
1:1000-1:5000
Immunohistochemistry-Paraffin
1:1000-1:5000~
Immunoprecipitation
1:50-1:200
Western Blot
1:1000-1:2000~
Application Notes
NOD2 produces, by alternative splicing, 13 different transcripts, all with introns, putatively encoding 13 different protein isoforms. Therefore, the molecular weights observed for Nod2 on Western blot may vary.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
50 ul neat whole antisera
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NOD2
Alternate Names
BLAU, CARD15caspase recruitment domain protein 15, caspase recruitment domain family, member 15, Caspase recruitment domain-containing protein 15, CDACUG, CLR16.3, IBD1NLR family, CARD domain containing 2, Inflammatory bowel disease protein 1, NLRC2, NOD-like receptor C2, nucleotide-binding oligomerization domain 2, nucleotide-binding oligomerization domain containing 2, nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 2, nucleotide-binding oligomerization domain-containing protein 2, PSORAS1NOD2B
Gene Symbol
NOD2
UniProt
Additional NOD2 Products
Product Documents for NOD2 Antibody
Product Specific Notices for NOD2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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