Nodal Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71686

Novus Biologicals, part of Bio-Techne
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NBP1-71686
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.32 mg/ml

View all Nodal Antibodies »

Product Summary for Nodal Antibody

Immunogen

A genomic peptide made to a C-terminal region of the human NODAL protein (within residues 200-347). [Swiss-Prot Q96S42]

Reactivity Notes

Immunogen has 98% identity to rat.

Localization

Secreted.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nodal Antibody

Western Blot: Nodal Antibody [NBP1-71686]

Western Blot: Nodal Antibody [NBP1-71686]

Western Blot: Nodal Antibody [NBP1-71686] - Analysis of NODAL in HeLa whole cell extracts.
Immunocytochemistry/ Immunofluorescence: Nodal Antibody [NBP1-71686]

Immunocytochemistry/ Immunofluorescence: Nodal Antibody [NBP1-71686]

Immunocytochemistry/Immunofluorescence: Nodal Antibody [NBP1-71686] - ICC staining of NODAL in HeLa cells.

Applications for Nodal Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500
Application Notes
In Western blot, a band is seen ~40 kDa.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 0.1% BSA, and 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

0.32 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nodal

NODAL is an important TGF-beta family protein in cellular differentiation. While constitutively expressed in many developing tissues, NODAL is problematically associated with the differentiation and development of many cancers. The expression of NODAL is regulated by a Notch signaling pathway, which when disrupted contributes to the aggressive behavior of metastatic cancer cells, particularly in melanoma. The abundance of NODAL expression in melanoma can help determine the stage of cancer and metastatic type/progression when tested in IHC. NODAL interactions with Cripto also predispose toward tumor progression. Overexpression of NODAL is also associated with preeclamptic placenta.

Alternate Names

BMP-16

Entrez Gene IDs

4838 (Human); 18119 (Mouse)

Gene Symbol

NODAL

UniProt

Q96S42, P43021

Additional Nodal Products

Product Documents for Nodal Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Nodal Antibody

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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